摘要
本实验对18个品种的高羊茅种子贮藏蛋白进行了十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）技术分析。结果表明：供试材料具有较丰富的贮藏蛋白等位变异，不同品种间Jaccard遗传相似系数GS变化范围为0.333~0.963；根据得到的遗传相似性矩阵进行非加权组法UPGMA聚类分析，建立高羊茅品种的系统树状图；以相似系数0.66为标准，可将所有品种分为2类。各品种的电泳图谱可以相互区别，表明种子贮藏蛋白SDS-PAGE电泳图谱可作为高羊茅品种鉴定的蛋白质指纹图谱。
In this study, we analyzed the seed storage protein of 18 cuhivars of tall fescue with using SDS-PAGE. The results show that high level of storage protein variations were presented in tall rescue, the range of Jaecard＇s genetic similarity~ coefficient among these varieties varied from 0.54 to 0.96. A protein dendrogram was established for 18 varieties of tall fescue based on UPGMA analysis of the genetic similarity matrix. Two groups were identified based on a standard of 0.66 similarity coefficient in the dendro- gram. All varieties of electrophoresis can be distinguished from each other, SDS-PAGE of seed storage protein can be regarded as protein fingerprints for identification of tall rescue varieties.
出处
《科技视界》
2012年第12期36-38,72,共4页
Science & Technology Vision
基金
长江大学科研基金的资助
关键词
高羊茅
贮藏蛋白
SDS-PAGE
电泳谱带
品种鉴定
Tall rescue
Storage protein
SDS-PAGE
Electrephoresis patterns
Cuhivar identification
