摘要
用苹果轮纹病菌菌株Ls1的菌丝接种苹果抗病品种‘红玉’和感病品种‘金冠’及其杂种后代高抗单株02-24-036和高感单株02-38-071的果实,采用扫描和透射电镜技术观察其表面及皮下组织亚显微结构的变化情况。结果发现,病菌侵染后,红玉、金冠和02-24-036果面均产生特殊的分泌物,其中红玉和02-24-036产生的分泌物抑制菌丝繁殖,金冠果面产生的分泌物不能抑制菌丝繁殖。说明病菌侵染后果面产生的分泌物与抑菌和抗侵入有关。菌丝由果面皮孔或伤口侵入组织后,抗病的红玉和02-24-036皮下细胞内嗜锇颗粒簇积累,而感病的金冠和02-38-071未见嗜锇颗粒簇的积累。说明红玉和02-24-036果实对苹果轮纹病的抗性属于诱导抗性,既表现为抗侵入,也表现为抗扩展。
The ultrastructural changes of the surface and subcutaneous tissue were observed through scanning and transmission electron microscopy using resistant varieties‘Jonathan’and susceptible cultivars ‘Golden Delicious’and their hybrid seedings（Jonathan × Golden Delicious）including highly resistant 02-24-036 and highly susceptible 02-38-071 as materials inoculated by the strong pathogenic Ls1 strain [Botryosphaeria dothidea（Moug.）Ces. et de Not.]. It found that parents and 02-24-036 all produced special secretions after infection. The secretions of Jonathan and 02-24-036 significantly inhibited the invasion and reproduction of hyphae on fruit surface,while secretions of Golden Delicious couldn’t do that. Results showed that these special secretions were connected with bacteriostasis and resistance to invasion. After hyphae invaded tissue through lenticel and wound,accumulation of osmiophilic globule was found in Jonathan and 02-24-036,while this phenomenon was not observed in Golden Delicious and 02-38-071. These results suggested that the resistance in Jonathan and 02-24-036 to apple fruit ring rot disease isinduced resistance,which performed both anti-intrusion and resistance to expansion.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第5期963-969,共7页
Acta Horticulturae Sinica
基金
国家现代苹果产业技术体系项目(CARS-28)
国家‘863’计划课题(2011AA001204)
北京市果树逆境生理与分子生物学重点实验室项目
关键词
苹果
果实
轮纹病菌
抗病性
Malus domestica
fruit
Botryosphaeria dothidea
resistance
