摘要
目的:研究肺岩宁方对Lewis肺癌小鼠CD4+CD25+调节性T细胞(T regulatory cell,Treg)比例及转录因子Foxp3表达的影响。方法:建立小鼠Lewis肺癌细胞移植瘤模型后,将32只6～8周龄雄性C57BL/6小鼠随机分为模型组、中药组、化疗组和综合治疗组。给予相应药物干预14d后,观察小鼠的一般状况、饲料消耗量、体质量、去瘤体质量、移植瘤质量、肺脏指数、脾脏指数和胸腺指数,并检测各组小鼠胸腺、脾脏和移植瘤CD4+CD25+Treg细胞的比例及Foxp3mRNA的表达。结果:肺岩宁方可降低肺癌小鼠脾脏和胸腺指数,增加去瘤体质量,减少移植瘤质量,与模型组比较差异有统计学意义(P<0.05)。肺岩宁方可显著降低Lewis肺癌小鼠胸腺、脾脏和移植瘤中的CD4+CD25+Treg细胞比例,并抑制Foxp3mRNA的表达,与模型组比较差异有统计学意义(P<0.05)。结论:肺岩宁方通过降低CD4+CD25+Treg细胞比例、下调Foxp3 mRNA表达而增强机体的抗肿瘤免疫应答发挥抑瘤作用。
OBJECTIVE： To study the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, on the ratio of CD4＋ CD25＋ regulatory T cells and expression of transcription factor Foxp3 in mice with Lewis lung cancer. METHODS： Thirty-two male wild-type C57BL/6 mice aging from 6 to 8 weeks were inoculated with Lewis lung cancer cells to establish the tumor-bearing model of Lewis lung carcinoma and were randomly divided into model group, Chinese medicine group, chemotherapy group and Chinese medicine combined with chemotherapy group. After intervention for 14 d with corresponding drugs, behaviors, physical signs and changes of feed consumption of the mice were observed. All mice were sacrificed after drug treatment, and tumors and organs were removed to weigh and calculate organ indexes （lung index, spleen index and thymus index）, The percentages of CD4＋ CD25＋ regulatory T cells in the thymus, spleen and tumor were determined by flow cytometry. The expression of Foxp3 mRNA in the thymus, spleen and tumor tissues was detected by quantitative real-time polymerase chain reaction. RESULTS： Compared with those in the model group, the mice in the Chinese medicine group showed significant reductions in spleen and thymus indexes and tumor weight, and elevation in the body weight without tumor （P〈0.05）. The numbers of CD4＋CD25＋ regulatory T cells in spleen, thymus and tumor were lower in the Chinese medicine group than in the model group （P〈0. 05）. The expression of Foxp3 mRNA in spleen, thymus and tumor was significantly down-regulated in the Chinese medicine group compared with the model group （P〈0.05）. There were no significant differences in CD4＋ CD25＋ regulatory T cell ratio and Foxp3 mRNA expression between the Chinese medicine combined with chemotherapy group and the chemotherapy group. CONCLUSION： Feiyanning Decoction can enhance the antitumor immune response and thus play a role in antitumor therapy by reducing the ratio of CD4＋ CD25＋ regulatory T cells and down-regulating the expression of Foxp3 mRNA.
出处
《中西医结合学报》
CAS
2012年第5期584-590,共7页
Journal of Chinese Integrative Medicine
基金
上海市科学技术委员会资助项目(No.09140903600)
上海市教育委员会资助项目(No.10YZ51)
