摘要
背景:前期实验证明红景天苷能诱导骨髓间充质干细胞向神经元样细胞定向分化,但是具体的影响方式尚不清楚。目的:以骨髓间充质干细胞为研究对象,进一步探讨红景天苷诱导其向神经元样细胞定向分化的作用方式和分子机制。方法:选取第2代Wistar大鼠骨髓间充质干细胞,按照不同的诱导剂分为维甲酸诱导组、红景天苷诱导组和对照组,通过不同的时间点对细胞进行观察。结果与结论:两诱导组细胞不同时间点神经干细胞标志物巢蛋白、神经细胞分化相关的兔抗微管蛋白和神经微管相关蛋白2表达阳性率均高于对照组(P<0.01);神经胶质纤维酸性蛋白阳性率维甲酸组显著高于红景天苷组(P<0.01)。RealTime-PCR结果显示,维甲酸和红景天苷诱导不同时间,两组细胞均有神经细胞相关基因神经元特异性烯醇化酶、神经微管相关蛋白2mRNA表达,与诱导时间有关且不完全相同,两组间神经胶质纤维酸性蛋白mRNA的高丰度出现于诱导早期;诱导后两组神经元特异性烯醇化酶蛋白的表达随时间延长显著增加,兔抗微管蛋白表达出现于诱导早期。说明红景天苷能促进骨髓间充质干细胞向神经细胞分化,其诱导效应与维甲酸相似,且向神经元样细胞定向分化方面优于维甲酸。
BACKGROUND：Preliminary experiments have demonstrated that salidroside（SD） can induce bone marrow mesenchymal stem cells（BMSCs） to differentiate into neural-like cells.However,little is known about how SD affects BMSCs differentiation into neural-like cells.OBJECTIVE：To investigate the molecular mechanism by which SD induces BMSCs to differentiate into neural-like cells.METHODS：Passage 2 Wistar rat BMSCs were divided into a retinoic acid（RA） group,SD group,and a control group.Cells were observed at different time points.RESULTS AND CONCLUSION：The expression levels of neural stem cell marker nestin,neural cell differentiation-related to rabbit anti-microtubule protein and microtubule-associated protein 2（MAP2） in the RA and SD groups were significantly higher than those in the control group at each time point（P 0.01）.The expression level of glial fibrillary acidic protein（GFAP） in the RA group was significantly higher than that in the SD group（P 0.01）.Real-time PCR results suggested that there were neuron-specific enolase（NSE） and MAP2 mRNA expressions in the RA and SD groups at each time point.The expression level depended but not absolutely on induction time.The high abundance of GFAP mRNA in the RA and SD groups appeared in the early stage of induction.NSE protein expression in the RA and SD groups was significantly increased with prolongation of induction time and rabbit anti-microtubule protein appeared in the early stage of induction.These findings suggest that SD,similar to RA,can promote BMSCs to differentiate toward neural cells,but it exhibits better effects on differentiation towards neural-like cells than RA.
出处
《中国组织工程研究》
CAS
CSCD
2012年第14期2496-2504,共9页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金面上项目资助(81073156)
课题名称:红景天苷诱导间充质干细胞向神经细胞分化的作用及分子机制~~
