摘要
背景:目前急性淋巴细胞白血病的病因尚未完全清楚,而CD4+CD25+T调节细胞对机体的免疫反应可能起着一定的调节作用。目的:观察急性淋巴细胞白血病患者外周血CD4+CD25+T调节细胞分泌白细胞介素2、白细胞介素10及转化生长因子β的情况。方法:采用密度梯度离心法分离22例B细胞系列的急性淋巴细胞白血病(B-ALL)患者、13例T细胞系列的急性淋巴细胞白血病(T-ALL)患者和18名正常人外周血单个核细胞。采用免疫磁珠法分离出CD4+CD25+T调节细胞进行培养,同时用双抗体夹心酶联免疫吸附试验检测细胞培养上清中白细胞介素2、白细胞介素10及转化生长因子β的水平。结果与结论:B-ALL患者和T-ALL患者外周血CD4+CD25+T调节细胞培养上清中白细胞介素10和转化生长因子β水平显著高于正常人水平,而白细胞介素2水平显著低于正常人水平(P<0.05);B-ALL和T-ALL患者的CD4+CD25+T调节细胞培养上清中3种因子表达差异无显著性意义(P>0.05)。提示CD4+CD25+T调节细胞可能通过增加分泌白细胞介素10和转化生长因子β、减少分泌白细胞介素2等干扰抗肿瘤的免疫反应。
BACKGROUND：At present,the cause of acute lymphoblastic leukemia（ALL） has not yet entirely understood,CD4 ＋ CD25 ＋ regulatory T cells（Treg） may have certain regulatory effect on immune response in pathogenic process of ALL.OBJECTIVE：To study the levels of interleukin 2（IL-2）,interleukin 10（IL-10）,transforming growth factor（TGF-β） of CD4 ＋ CD25 ＋ regulatory T cells（Treg） in peripheral blood of patients with ALL.METHODS：The peripheral blood mononuclear cells（PBMC） were isolated with density gradient centrifugation from 22 B cell series ALL（B-ALL） patients,13 T cell series ALL（T-ALL） patients and 18 normal people.Immunomagnetic bead separation was used to separate CD4 ＋ CD25 ＋ Treg cells,and the levels of IL-2,IL-10 and TGF-β in the cells culture supernatant were detected by using enzyme-linked immunosorbent assay（ELISA）.RESULTS AND CONCLUSION：The levels of IL-10 and TGF-β in the cells culture supernatant of B-ALL patients and T-ALL patients were significantly higher than those in normal people,while the level of IL-2 was significantly lower than that in normal people（P 0.05）.There was no significant difference between B-ALL and T-ALL patients in the IL-2,IL-10 and TGF-β levels of the cells culture supernatant（P 0.05）.CD4 ＋ CD25 ＋ Treg cells might interfere the immunologic reaction of antitumor by increasing the IL-10 and TGF-β levels and decreasing the IL-2 level.
出处
《中国组织工程研究》
CAS
CSCD
2012年第14期2648-2651,共4页
Chinese Journal of Tissue Engineering Research
基金
海南省卫生厅科研立项课题(琼卫2008-55)~~
