摘要
通过对毛木耳原生质体制备与再生条件的系统研究,确定了原生质体制备的最佳出发菌龄、融壁酶浓度、酶解温度及酶解时间、渗透压稳定剂种类、浓度及最佳再生培养基配方。
The preparation conditions of Auricularia polytricha protoplasts were studied by the type of liquid cultural medium, age of mycelium, type and concentration of lywallzyme, temperature and time of enzymolysis, type and concen- tration of osmotic stabilizer and regeneration medium.
出处
《中国食用菌》
2012年第3期35-37,共3页
Edible Fungi of China
基金
国家现代农业产业技术体系
关键词
毛木耳
原生质体
制备
再生
A uriculariapolytricha
Protoplast
Preparation
Regeneration