摘要
目的:检测不同胃疾病黏膜中胃蛋白酶原(pe-psinogen C,PGC)的表达及血清sPGC、sPGA浓度,分析二者的相关性,给临床工作提供更有益信息.方法:免疫组织化学染色法检测不同胃疾病黏膜组织中PGC的表达,并结合ELISA法检测血清中sPGC、sPGA的含量.之后用SPSS16.0分析软件进行统计学处理,P<0.05具有统计学意义.结果:PGC在不同胃疾病黏膜中的表达有差异(P=0.000).浅表-萎缩-伴肠化-伴异型增生-腺癌,PGC阳性率呈现递减.sPGC、sPGA浓度在各组间比较差异有统计学意义(P=0.000,P=0.000).sPGA呈现递减,浅表组与其余各组相比差异有统计学意义(P=0.035,0.000,0.000,0.000);萎缩组与其余各组相比差异有统计学意义(P=0.000,0.000,0.031);肠化组/异型增生组与胃癌组比较差异有统计学意义(P=0.047,0.034);sPGC表现为逐渐上降趋势,胃癌组与其他各组相比差异显著(P=0.000,0.000,0.003,0.001).浅表-萎缩-肠化-异型增生-胃癌,PGC表达与sPGA呈负相关,与sPGC呈正相关(r=0.956,P=0.011vssPGA;r=-0.968,P=0.007vssPGC).结论:PGC的动态表达与胃疾病的发生发展有良好的相关性;血清sPGA明显降低提示可能与胃癌的发生发展有关;若同时进行血清PG值及组织PGC抗原表达率检测,二者具有良好的相关性,可作为临床筛查胃癌及癌前疾病的指标.
AIM：To detect the gastric expression levels of pepsinogen C （PGC） and serum levels of sPGA and sPGC in patients with various gastric diseases and to analyze their correlation.METHODS：Gastric PGC were measured by immunohistochemistry,and serum levels of sPGA,sPGC were measured by ELISA in patients with various gastric diseases,respectively.The data were analyzed for significance using the SPSS16.0 software.RESULTS：There were significant differences in gastric PGC expression among patients with different gastric diseases （P=0.000）.The positive rate of PGC expression was highest in patients with superficial gastritis （SG）,followed by those with atrophic gastritis （AG）,intestinal metaplasia （IM）,dysplasia （DYS） and gastric carcinoma （Ca）.The positive rate of PGC was significantly higher in SG than in other lesions （P=0.035,0.000,0.000,0.000）,in AG than in IM,DYS and Ca （P=0.000,0.000,0.031）.There were also significant differences in serum levels of sPGA and sPGC among different patient groups （both P=0.000）.Similar to PGC expression,serum levels of sPGA also decreased in an order of SG-AG-IM-DYSCa.In contrast,serum levels of sPGC in Ca were significantly higher than those in other lesions （P=0.000,0.000,0.003,0.001）.The positive rate of PGC expression had a positive correlation with serum levels of sPGA and a negative correlation with serum levels of sPGC （r=0.956,P=0.011 vs sPGA;r=-0.968,P=0.007 vs sPGC）.CONCLUSION：Tissue expression of PGC is negatively associated with the malignant degree of gastric mucosa cells and positively with the development of gastric mucosal diseases.Combined detection of sPG and PGC expression can help screen and diagnose gastric mucosal diseases.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第14期1242-1245,共4页
World Chinese Journal of Digestology
