摘要
2006年,研究人员将4个转录因子(Oct4、Sox2、cMyc和Klf4)通过逆转录病毒导入已分化的小鼠皮肤成纤维细胞,进而获得了类似于胚胎干细胞的多能性干细胞,称之为"诱导性多能干细胞"(Induced pluripotent stem cells,iPS cells)。这种方法可稳定地将高度分化的成体细胞重编程为具有多能性的干细胞,应用于疾病治疗,可避免传统胚胎干细胞应用所受到的伦理道德等社会因素的限制。因此,该技术的诞生在很大程度上推进了干细胞理论及临床应用研究的发展。目前,有多种途径诱导体细胞重编程为iPS细胞,本文就近年iPS细胞重编程的诱导方式作一简要综述。
In 2006, scientists obtained a kind of pluripoteney cells, named as induced pluripotent stem cells （iPS cells） by importing four transeription factors （Oct4, Sox2, eMye and Klf4） into differentiated mouse fibroblast cells. Differentiated cells can be reprogramed to pluripotent stem cells stably with this technology and used for treatment of diseases. In this way, the limitation of ap- plication of traditional embryonic stem cells by soeial factors, such as ethical and moral factors, may be avoided. Therefore, the de- velopment of the method promotes the progress in research on theory and clinical application of stem cells enormously. This paper reviews the methods for inducing reprograme of iPS cells developed in recent years.
出处
《中国生物制品学杂志》
CAS
CSCD
2012年第5期653-656,共4页
Chinese Journal of Biologicals
关键词
多能干细胞
重编程
诱导方式
Pluripotent stem cells
Reprogramme
Method for induction
