摘要
目的探讨麻黄素对仔鼠肝组织结构、Bax蛋白的表达和超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力、丙二醛(MDA)含量及血清中谷草转氨酶(GOT)和谷丙转氨酶(GPT)活力的影响。方法对48只仔鼠采用递增剂量腹腔连续注射麻黄素溶液(2.0g/L、3.0g/L和4.0g/L)和等量的生理盐水,分别于注射5d、10d和15d称量检测仔鼠肝重的变化,用比色法测定仔鼠肝组织中SOD、CAT活性、MDA含量及血清中GOT和GPT活性的变化,用光学显微镜观察肝脏组织结构的变化,用免疫组织化学法检测肝组织中Bax蛋白表达的变化。结果注射麻黄素5d、10d、15d仔鼠的肝重低于对照组;随着给药时间的延长和药剂量的增加,SOD和CAT的活性先升高后降低,实验组仔鼠5d时SOD、CAT的活性高于对照组,差异显著(P<0.05),10d、15d时SOD、CAT的活性低于对照组,差异显著或极显著(P<0.05或P<0.01);MDA含量则先降低后升高,实验组仔鼠5d时MDA含量低于对照组,差异显著(P<0.05),10d、15d时MDA含量高于对照组,差异极显著(P<0.01)。注射麻黄素后仔鼠血清内GOT和GPT活性高于对照组,差异显著或极显著(P<0.05或P<0.01)。注射麻黄素后仔鼠肝脏有不同程度的损伤,肝板萎缩,肝血窦扩张,细胞界限模糊,内皮细胞脱落;Bax蛋白阳性表达数高于对照组,差异显著或极显著(P<0.05或P<0.01)。结论麻黄素影响发育期仔鼠肝脏的组织结构,这可能与肝组织中抗氧化物酶活性降低和MDA含量升高有关。
Objective To investigate changes of structures, expression of Bax protein activities of superoxide dismutase（SOD） and catalase（CAT） , and contents of maleic dialdehyde（MDA） in the liver of filial mice and activity of glutamic-oxalacetic transaminease （GOT） and glutamic-pyruvic transaminase （GPT） in serum after injected ephedrine. Methods Totally 48 filial mice were used in this study. They were intraperitoneally injected with escalation doses （ 2. 0g/L, 3.0g/L and 4. 0g/L ） ephedrine and the same amount of saline. Changes of the liver weight were examined. Activities of SOD, CAT, GOT, GPT as well as content of MDA were determined by calorimetry. Hepatic structures of 5, 10 and 15 days filial mice were observed by optical microscopy after injected ephedrine and the expression of Bax protein was measured by immunohistoehemical staining. Results The liver weight of filial mice of the experimental groups was lower than the control groups at day 5 10 and 15 after injected ephedrine. The activity of SOD and CAT increased in the early ages and, then decreased with the extension of time and the increasing doses of the drug, SOD and CAT activities of the experimental groups were higher at day 5 （ P 〈 0. 05 ） and lower at day 10 （ P 〈 0.05 ） and day 15 （ P 〈 0. 01 ） than that of the control group. While MDA content decreased firstly and then increased, MDA content of the experimental group lower than the control group at day 5 （ P 〈 0. 05 ） , and higher than the control group at day 10 and 15 （ P 〈 0.01 ）. The activity of GOT and GPT in filial mice serum of the experimental groups were higher than the control groups after injection of ephedrinc（P 〈 0. 05 or P 〈 0. 01 ）. Liver of filial mice appeared various degrees of damage after ephedrine injection,such as hepatic plate atrophy, hepatic sinusoid expand, the cell boundaries fuzzy and endotheliocyte fall off. The intensity of the expression of Bax protein of the experimental groups was higher than the control group after ephedrine injection（ P 〈 0. 05 or P 〈 0. 01 ）. Conclusion The ephedrine affects the liver of developmental filial mice. This damage may be correlated with the low activities of SOD, CAT and high content of MDA in the filial mice.
出处
《解剖学报》
CAS
CSCD
北大核心
2012年第3期398-404,共7页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30370163)
甘肃省自然科学基金资助项目(1107RJZA141)
甘肃省证据科学技术研究与应用重点实验室科技计划资助项目(ZDSY-Y08)
兰州市社会发展基金资助项目(2010-127)
关键词
麻黄素
肝脏
BAX蛋白
酶活性
比色法
仔鼠
Ephedrine
Liver
Bax protein
Enzyme activity
Colorimetry
Filial mouse
