摘要
目的:建立同时测定绿茶中表没食子儿茶素没食子酸酯(EGCG)和咖啡因含量的方法。方法:采用高效液相色谱法。色谱柱为DiamonsilTMC18(200mm×4.6mm,5μm),流动相为甲醇-0.1%冰醋酸水溶液(25:75),检测波长为278nm,流速为1.0mL.min-1,柱温为30℃。结果:EGCG和咖啡因的进样量分别在1.25～10.00μg(r=0.9994)和0.25～2.00μg(r=0.9996)范围内与各自峰面积积分值呈良好的线性关系;平均加样回收率分别为98.06%和99.37%,RSD分别为1.05%和1.44%(n均为6)。结论:该方法简便、准确、重复性好,可用于绿茶的质量控制。
OBJECTIVE： To establish the method for the content determination of epigallocatechin gallate （EGCG） and caffeine in green tea simultaneously. METHODS： HPLC method was adopted. A Diamonsil^TMC18（200 mm×4.6 mm, 5 μm） column was used with mobile phase consisted of methanol-0.1% acetic acid （25：75） at the detection wavelength of 278 nm and flow rate of 1.0 mL.min^-1. The column temperature was 30 ℃. RESULTS： The linear ranges were 1.25-10.00 μg for EGCG（r=0.999 4）, 0.25- 2.00 μg for caffeine （r=0.999 6）. The average recoveries were 98.06% and 99.37%, respectively. RSDs were 1.05% and 1.44% （n=6）, respectively. CONCLUSION： The method is simple, accurate and highly reproducible, and can be used for the quality control of the green tea.
出处
《中国药房》
CAS
CSCD
2012年第23期2178-2179,共2页
China Pharmacy
