摘要
目的提供一种去除聚合酶链式反应(PCR)引物二聚体的简便方法。方法根据聚乙二醇(PEG)能够选择性沉淀DNA片段的原理,确定分别去除100 bp、100～200 bp、100～300 bp条带的PEG 6000最终质量分数,因引物二聚体大小一般为200 bp,最终运用到去除含有引物二聚体的PCR产物中。结果最终质量分数为10.67%、9.00%、8.00%的PEG能分别除去100 bp、100～200 bp、100～300 bp条带。最终质量分数为24.00%的PEG能有效去除PCR引物二聚体并纯化PCR产物。结论 PEG 6000能有效去除PCR扩增产物的引物二聚体,达到纯化PCR产物的效果。
Objective To provide a simple method of removing primer dimers produced by the polymerase chain reaction（PCR）. Methods According to the principle that polyethylene glycol（PEG） had been used to precipitate DNA fragments selectively,the final concentrations of PEG removing 100 bp,100-200 bp and 100-300 bp DNA fragments were determined.And PEG was used to remove PCR primer dimers finally because the size of the primer dimmers was 200 bp. Results The straps of 100 bp,100-200 bp and 200-300 bp were removed by PEG with the concentrations of 10.67%,9.00% and 8.00%,respectively.The PCR primer dimmers could be removed and the PCR products could be purified effectively by a final concentration of 24.00% PEG. Conclusion PEG 6000 can be used to remove the primer dimer effectively and purify the PCR products simultaneously.
出处
《新乡医学院学报》
CAS
2012年第6期417-418,421,共3页
Journal of Xinxiang Medical University
基金
河南省科技厅科技攻关项目(编号:0624410041)
河南省教育厅自然科学基金项目(编号:2008A310008)
