摘要
近年来,土壤环境受到越来越严重的污染,其中一个很重要的原因是农药的滥用造成的,目前农药对土壤动物(尤其是蚯蚓)的影响受到极大重视并成为研究热点。为评价土壤的农药污染程度,选用乐果和杀虫双及其二者复合对蚯蚓进行染毒试验,采用滤纸法研究不同浓度农药污染对赤子爱蚯蚓体内蛋白质含量的影响。结果表明:蚯蚓体内蛋白质含量在乐果污染24 h和48 h均比对照显著增加(P<0.05),随着乐果浓度的升高,蚯蚓体内蛋白质含量呈现出先增加后降低的趋势,而随杀虫双浓度的升高,蛋白质含量的影响没有明显的规律性;不同浓度处理之间差异显著(P<0.05),同一浓度处理24 h和48 h之间有极显著的差异(P<0.01);固定乐果浓度(60 mg/kg)分别和不同浓度杀虫双处理24 h和48 h,蚯蚓体内蛋白质含量随着杀虫双浓度的升高,均呈现出升高—降低—升高的锯齿型变化曲线,各复合处理之间有显著差异(P<0.05);回归分析结果表明,蚯蚓体内蛋白质含量与乐果和杀虫双浓度存在一定剂量-效应关系,其关系曲线均为抛物线型,乐果对蚯蚓体内蛋白质含量的影响高于杀虫双。因此可利用蚯蚓体内蛋白质含量的变化较好的指示乐果对蚯蚓的毒性作用,对杀虫双的毒性作用指示效果较乐果差。
The content of earthworm＇s esoteric protein increased significantly compared with that of the control（P0.05） after contaminated by dimethoate 24 hours and 48 hours respectively,with the dimethoate concentration increasing,earthworm＇s esoteric protein content increased first and then decreased,while it had no obvious regularity in the increase of dimehypo＇s concentration.The effects of different concentrations on the content of protein was significant（P0.05）.Treated for 24 hours and 48 hours with the same concentration the different was significant（P0.01）;Fixed dimethoate concentration（60 mg/kg） combined with different concentrations of dimehypo respectively,the content of earthworm＇s esoteric protein showed zigzag curves（increasing-descending-increasing） treated for 24 hours or 48 hours with the dimethoate concentrations increasing,there was significant difference between different composite treatments（P0.05）.Regression analysis showed that the content of earthworm＇s esoteric protein was related with the concentrations of dimethoate and dimehypo to some extent,and its＇ relationship appeared as a parabolic curve,the effect of dimethoate on earthworm＇s protein content was greater than that of dimehypo.Therefore,the change of protein content of earthworms can be used to indicate the toxicity of dimethoate on earthworms,the indicative effect of dimehypo＇s toxicity was poorer than that of dimethoate.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2012年第2期298-303,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
云南省科技厅省院省校合作项目(2006yx35)
云南省"十五"科技攻关项目(2002NG03)
关键词
乐果
杀虫双
蚯蚓
蛋白质含量
dimethoate
dimehypo
earthworm
protein content
