摘要
研究了用毛细管区带电泳法快速测定复方新诺明片中磺胺甲口恶唑 ( SMZ)和甲氧苄氨嘧啶 ( TMP)的含量。使用长 2 7cm(有效长度 2 0 cm)内径 50 μm的石英毛细管 ,2 5k V分离电压 ,在 0 .0 5mol/L的乙酸盐缓冲液 ( p H5.5)中 ,上述两组分可在 1 .5min内完全分离。用紫外检测器在 2 1 4nm处检测 ,外标法定量。 1 0次测定含有 1 41 .7mg/L SMZ和 2 5.6mg/L TMP的试样溶液 ,相对标准偏差为 1 .0 % ( SMZ)和 0 .8% ( TMP) ,回收率为 98.0 % ( SMZ)和 1 0 1 .0 % ( TMP) ,检出限 ( 3σ)分别为 3.5mg/L( SMZ)和 1 .1 mg/L( TMP)。建立的方法测定复方新诺明片剂中两组分的含量 。
A capillary zone electrophoresis(CZE) method for fast determination of sulfamethoxazole and trimethoprim in sulfatrim tablets has been developed. Using a 27 cm(effective length 20 cm)×50 μm i.d. fused quartz capillary, CZE was performed in 0.05 mol/L acetate buffer solution(pH 5.5) under the applied voltage of 25 kV.The two active ingredients were completely separated within 1.5 minutes, and quantified with a UV detector at 214 nm by external standard calibration. Relative standard deviations of 1.0 % for sulfamethoxazole and 0.8 % for trimethoprim were achieved for 10 determinations of a sample solution containing 141.7 mg/L sulfamethoxazole and 25.6 mg/L trimethoprim,and recoveries of 98% and 101% were obtained respectively for spiked sulfamethoxazole and trimethoprim. Detection limits(3σ) were 3.5 mg/L for sulfamethoxazole and 1.1 mg/L for trimethoprim. The proposed method has been applied for the analysis of sulfatrim tablets,and the analytical results are in good agreement with those obtained by dual wavelength spectrophotometer method regulated by Chinese Pharmacopoeia.
出处
《分析科学学报》
CAS
CSCD
2000年第2期123-126,共4页
Journal of Analytical Science
基金
浙江省自然科学基金
关键词
复方新诺明片
定量分析
抗菌消炎药
电泳法
Capillary zone electrophoresis
Sulfamethoxazole
Trimethoprim
Sulfatrim tablet
Quantitative analysis
