摘要
目的:研究海藻多糖(Polysaechrides from Spirulina Platensis,PSP)对小鼠肝损伤动物模型与肝匀浆脂质过氧化模型的抗氧化作用。方法:采用腹腔注射CCL4的方法,制造小鼠肝损伤动物模型,金氏法测定血清谷氨酸丙酮酸转氨酶(SGPT)和肝脏的过氧化脂质(Lipoper-oxides,LPO)含量;Fenton反应诱导小鼠肝匀浆脂质过氧化动物模型,采用硫代巴比妥酸(TBA)法测定肝匀浆LPO。结果:在体外,海藻多糖可剂量依赖性地抑制Fenton反应导致的CCL4毒化小鼠肝匀浆中LPO的生成,其IC50为1.6×10-4。在体内,灌胃250mg/kg的海藻多糖组可明显降低CCL4毒化小鼠血清SGPT活性(P<0.01)。结论:海藻多糖对体内外的肝组织有一定程度的抗氧化作用。
Objective To study the antioxidant effects of the seaweed polysaccharides（Polysaechrides from Spirulina Platensis,PSP） on liver injury in animal models and liver homogenate lipid peroxidation models.Methods The Intraperitoneal injection of CCL was conducted to create animal models of liver injury in mice;Kim method was taken to measure the content of serum glutamate pyruvate transaminase（SGPT） and liver lipid peroxidation（Lipoperoxides,LPO）;Fenton reaction induced mouse liver homogenate lipid peroxidation in animal models.The thiobarbituric acid（TBA） method was adopted for the determination of liver homogenate LPO.Results In vitro,seaweed polysaccharides dose-dependently inhibited LPO generation in mice liver homogenate poisoned by CCL4,which was resulted from the Fenton reaction.IC50= 1.6×10-4.In vivo,the fed 250mg/kg of seaweed polysaccharide group can significantly reduce the SGPT activity of CCL4 in poisoning the mouse serum（P0.01）.Conclusion Seaweed polysaccharides has a certain degree of anti-oxidation on liver tissue in vitro and in vivo.
出处
《宜春学院学报》
2012年第4期106-107,119,共3页
Journal of Yichun University
