摘要
[目的]对黑钙土中真菌的时空分布及其在贫营养条件下菌落特征进行研究。[方法]采用稀释平板法对真菌进行分离,用单孢子分离法进行纯化,用不同稀释浓度的察贝克培养基进行培养计数。[结果]从供试黑钙土中分离出9种真菌菌属,并且确定了4种优势菌属,同时发现黑钙土中真菌数量分布呈现出明显的季节性,并且随采样深度增加其数量呈现减少的趋势,在贫营养条件下真菌种类和数量均明显减少。[结论]该研究可为黑钙土中微生物的相关研究提供理论依据。
[ Objective ] The aim was to study the spatial-temporal distribution of fungi in chernozem and its colony features under oligotrophic conditions. [ Method] The fungi was firstly separated with the fiat plate dilution, and then purified by single spore separation method, and finally cultured and counted in Czapek-Dox medium which was diluted into different concentrations. [ Result ] A total of nine genera of fungi were isola- ted from the tested chernozem, and four of them were identified to be dominant genera. The distribution of fungi quantity showed an obvious sea- sonal variation in chernozem,and it decreased with the sampling depth. Under the oligotrophic conditions, both the species and the quantity of fungi decreased significantly： [ Conclusion] The research provided theoretical basis for the related research of microbes in chernozem.
出处
《安徽农业科学》
CAS
2012年第14期8136-8138,共3页
Journal of Anhui Agricultural Sciences
关键词
黑钙土
真菌
贫营养
Chernozem
Fungi
Oligotrophic
