摘要
目的:荧光量子点作为一种新型的荧光纳米材料,被广泛应用于生物医学基础研究领域,但这种纳米材料在生物体内代谢情况尚不清楚,在临床应用之前,需对其在体内的动态分布蓄积进行观测,系统评价其在吸收、转运及代谢过程中会对生物个体、组织、细胞甚至生物分子行为带来的影响以及毒副作用。方法:本文选取六周龄的雌性昆明小鼠为实验动物,将100μL浓度为289 nmol/L的CdSe/CdS/ZnS荧光量子点经尾静脉注射进入小鼠体内血液循环,分别处理1天、7天、14天后,处死小鼠,统计主要脏器重量变化,并制作组织涂片和石蜡切片。结果:组织涂片结果表明,各脏器中的量子点呈现动态分布。作用1天后,脾脏、肝脏、肺、心脏、肾脏和卵巢中都有量子点的分布,其中脾脏中最多;作用量子点7天后,脾脏蓄积减少,而肺部蓄积增多,肝、肾、卵巢蓄积量变化不大;作用14天后,各脏器中量子点明显减少,心脏和肾脏已无量子点蓄积,提示量子点已经从这些脏器中清除了。作用14天后,小鼠卵巢中仍存在量子点,提示量子点可能会在对雌性动物的生殖系统中滞留并造成生殖毒性。结论:石蜡切片结果表明,量子点在各脏器的蓄积未产生明显的病理性损伤。
Objective： Quantum dots（QDs）,as a type of novel nanocrystal luminescent materials,can be used widely in various basic biomedical investigations.However,we know little about the metabolism of QDs in vivo.Before applying them in clinics,we need to observe the in vivo dynamic biodistribution and bioaccumulation,and evaluate systematically the effects and toxicity of QDs on experimental animals,tissues,cells and even behavior of biomolecules during uptake,transportation and metabolism.Methods： In this work,100 μL CdSe/CdS/ZnS luminescent quantum dots solution was injected into 6 weeks Kunming female mice by tail intravenous injection.After treatment for 1 day,7days and 14days,mice were killed and the main organs were harvested,weighted and imaged,respectively.Results The tissue smears showed that QDs dynamically distributed in liver,spleen,lung,kidney,heart and ovary.After treatment for 1day,QDs distributed in all main organs,spleen,liver,lung,heart,kidney and ovary,and spleen accumulated most.After treatment for 7days,accumulation of QDs decreased dramatically in spleen,increased in lung and no obvious changes in liver,kidney and ovary.After treatment for 14 days,accumulation of QDs decreased in all organs,and there were not QDs in heart and kidney,which suggested that QDs had been cleared in heart and kidney.Notably,QDs were also found in ovary after treatment for 14 days,which suggested that QDs remained in ovary and have probably toxicity for reproductive system of female animal.Conclusions： Paraffin slices demonstrated that no obvious pathological damage were found in main organs.
出处
《中国医学物理学杂志》
CSCD
2012年第3期3423-3426,共4页
Chinese Journal of Medical Physics
基金
国家自然科学基金(No.30900335)
广东省自然科学基金(No.2008078)
深港创新圈专项资助计划(No.CXQ2008043)
关键词
量子点
分布和蓄积
组织损伤
组织涂片
quantum dots
biodistribution and bioaccumulation
damage of tissue
tissue smear
