摘要
背景:酸性成纤维细胞生长因子对缺血及再灌注心肌具有较好的保护作用,删去N端第21～27位的氨基酸序列后的改构型酸性成纤维细胞生长因子诱导的细胞增殖能力明显降低。目的:观察改构型酸性成纤维细胞生长因子对心肌缺氧/复氧后细胞凋亡的影响。方法:利用原代培养的SD新生大鼠心肌细胞建立体外心肌缺氧/复氧模型,分别用酸性成纤维细胞生长因子和改构型酸性成纤维细胞生长因子对此细胞模型进行干预。锥虫蓝拒染法检测心肌细胞存活率,流式细胞术检测心肌细胞凋亡率。结果与结论:改构型酸性成纤维细胞生长因子及酸性成纤维细胞生长因子均能显著降低缺氧/复氧后心肌细胞存活率及凋亡率(P<0.01)。证实,改构型酸性成纤维细胞生长因子能有效抑制缺氧/复氧后心肌细胞凋亡。
BACKGROUND： Acidic fibroblast growth factor （aFGF） can protect cardiomyocytes from ischemia/reperfusion damage, but the proliferative ability of the cells induced by modified aFGF （maFGF） is decreased. OBJECTIVE： To study the effect of aFGF and maFGF on cardiomytes apoptosis after anoxia/reoxygenation. METHODS： Myocardial anoxia/reoxygenation models with primary cultured cardiomyocytes from neonatal SD rats were established and then treated with aFGF and maFGF. The apoptosis rate of cardiomyocytes was detected by flow cytometry assay and the survival rate of cardiomyocytes was measured by typan blue exclusion. RESULTS AND CONCLUSION： Pretreatment with aFGF and maFGF decreased the apoptosis rate of cardiomyocytes after anoxia/reoxygenation （P 〈 0.01）. It has been proved that aFGF and maFGF have notable effects on attenuating cardiomyocyte apoptosis after anoxia/reoxygenation.
出处
《中国组织工程研究》
CAS
CSCD
2012年第20期3703-3706,共4页
Chinese Journal of Tissue Engineering Research
基金
国家863计划资助项目(2002AA223318)~~
