摘要
以多苯并咪唑配体1,1,4,7,7-五(2-苯并咪唑甲基)-二乙基三胺(DTPB)为主配体,合成了锰(Ⅱ)配合物[Mn(DTPB)Ac]Ac.8H2O(1)和[Mn2(DTPB)(NO3)2(H2O)2][Mn2(DTPB)(NO3)2(H2O)(CH3OH)].(NO3)4.5CH3OH.H2O(2),并对其进行了表征.利用紫外-可见吸收光谱和黏度实验研究了配合物1和2与DNA的相互作用,发现这2个配合物均能与DNA结合,并对配合物与DNA作用的机理进行了探讨.利用琼脂糖凝胶电泳和直角光散射(RALS)技术研究了配合物1和2促进DNA凝聚的性质.结果表明,在近中性条件下2个配合物都能促使DNA凝聚.利用透射电子显微镜(TEM)观察了不同凝聚体的形态.
Complexes [ Mn ( DTPB ) Ac ] Ac · 8H2O ( 1 ) and [ Mn2 ( DTPB ) ( NO3 )5 ( H2 O)2 ] [ Mn2 ( DTPB )- ( NO3 )2· ( H2O)· ( CH3 OH) ] ( NO3 )4-5CH3 OH· H20(2) were synthesized via 1,1,4,7,7-pentakis ( 1H-bcn- zimidazol-2-ylmethyl)-1,4,7-triazaheptane (DTPB) as the main ligand and characterized by X-ray diffraction. The interaction between complexes 1 or 2 and DNA, examined by UV-Vis absorption spectra and viscosity experiments, indicated that the two Mn ( I1 ) complexes have strong affinity for DNA. The interaction mechanism of the complexes with DNA was discussed. These Mn ( 11 ) complexes can promote DNA condensation under neutral conditions, as illustrated by gelose gel electrophoresis and right angle hght scattering (RALS). The morphologies of the DNA condensates were observed by transmission electron microscopy (TEM).
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2012年第6期1151-1157,共7页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:20971049
21001047)
华中师范大学博士基金(批准号:CCNU10A01010)资助