摘要
对河南省鸡源大肠杆菌Ⅰ类、Ⅱ类和Ⅲ类整合子及其携带的耐药基因盒进行了分子流行病学研究。结果表明:51株鸡源大肠杆菌中86.3%(44/51)检出Ι类整合子,3.9%(2/51)检出Ⅱ类整合子,未检出Ⅲ类整合子。Ⅰ类整合子共检出5种类型的基因盒,分别是sat基因盒(100%),dfrA1+aadA1基因盒(45.4%,20/44),dfrA17+aadA5基因盒(22.5%,9/44),dfrA1+sat+aadA2基因盒(6.8%,3/44)和4800bp未知基因盒(27.3%,12/44),其中4 800bp未知基因盒的下游携带有ESBLCTX-M基因,Ⅱ类整合子的基因盒携带dfrA1+sat+aadA13种基因。
An epidemiology investigation on integrons and gene cassettes in 51 Escherichia coli strains isolated from chicken of Henan province by PCR,restriction enzy analysis, sequence, and contrast. The results showed that forty- four stains（86.3%） harbored class Ⅰ integrons, two strains contained class Ⅱ integrons, and none of the isolates carried class Ⅲ integrons. Five types gene cassettes were detected in class Ⅰ integrons, including sat cassette （100%）, dfrA1 ＋ aadA1 cassette （45.4%,20/44 ）, dfrA17 ＋ aadA5 cassette （22. 5%, 9/44）, dfrA1 ＋ sat ＋ aadA2 cassette （6.8%, 3/44） and 4 800 bp unknown cassette （27. 3%, 12/44）. The ESBLCTX-M gene located in the downstream of 4 800 bp unknown cassette. The class Ⅱ integron harbored dfrAl＋sat＋aadA1 cassette.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第6期844-847,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30771624)
河南省科技攻关基金资助项目(102102110034)
