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Cd^(2+)与Zn^(2+)的联合效应对马氏珠母贝(Pinctada martensii)受精率的影响 被引量:2

COMBINED EFFECTS OF Cd^(2+) AND Zn^(2+) ON THE FERTILIZATION RATE IN PINCTADA MARTENSII(DUNKER)
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摘要 采用中心组合设计和响应曲面法在实验室条件下研究了Cd2+与Zn2+对马氏珠母贝受精率的影响。Cd2+、Zn2+的浓度范围分别为0.030—20mg/L、0.100—20mg/L。结果表明:Cd2+与Zn2+两因子对马氏珠母贝受精率的一次效应极显著(P<0.01);Cd2+与Zn2+两因子一次互作效应极显著(P<0.01)。经响应曲面法分析,随着Cd2+与Zn2+浓度的增加受精率呈下降趋势,当Zn2+浓度在最低浓度(0.100mg/L)时,受精率随着Cd2+浓度的上升而下降;当Cd2+浓度在最低浓度(0.030mg/L)时,受精率随Zn2+浓度的上升而先下降后升高。本实验建立了马氏珠母贝受精率与Cd2+、Zn2+间关系的模型方程(R2=0.996,Adj.R2=0.987,Pred.R2=0.887,P<0.01)并可用于预测Cd2+、Zn2+对马氏珠母贝受精率的影响。 Using the central composite face-centered design (CCF) and response surface methodology, the combined effects of Cdz+ (0.030--20.000mg/L) and Zn2+ (0.100--20.000mg/L) on the fertilization rate in Pinctada martensii were studied under laboratory conditions. The results showed that the effects of two factors Cd2+ and Zn2+ on the fertilization rate in P. martensii were highly significant (P〈0.01), and effect of Cd2+ was more significant than that of Zn2+. The interaction between the two factors was also highly significant (P〈0.01). Based on the response surface plots, it was found that with the increased concentration of Cd2+ and Zn2+ , the fertilization rate was decreased. When Zn2+ concentration was at its lowest level (0.100mg/L), the fertilization rate decreased with the increased Cd2+ concentration. When Cd2+ concentration was at its lowest level (0.030mg/L), the fertilization rate was first decreased and then increased as the concentration of Zn2+ increased. The model equation of the fertilization rate towards Cd2+ and Zn2+ was established, with R2 = 0.996, Adj.RE = 0.987 and Pred.R2 = 0.887 (P〈0.01). The model equation could be practically used for forecasting the effects of Cd2+ and Zn2+ on the fertilization rate in P. martensii.
出处 《海洋与湖沼》 CAS CSCD 北大核心 2012年第2期323-328,共6页 Oceanologia Et Limnologia Sinica
基金 广东省教育厅工程中心建设项目 GCZX-A0909号 广东省科技厅科技计划重大专项 2010B020201014号 科技部国家星火计划项目 2011GA780001号
关键词 马氏珠母贝 受精率 Cd2+ Zn2+ Pinctada martensii (Dunker), Fertilization rate, Cd2+, Zn2+
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