摘要
目的建立铁皮石斛叶中黄酮碳苷类成分HPLC指纹图谱,并同时测定主要成分芹菜素-6-C-α-L-阿拉伯糖-8-C-β-D-木糖苷的含量,为科学评价和有效控制其质量提供依据。方法采用XB C18色谱柱(4.6 mm×250 mm,5μm),甲醇-0.4%甲酸水溶液为流动相,梯度洗脱,检测波长335 nm。收集了14批铁皮石斛叶和3批同属不同种石斛叶进行测定,用国家药典委员会"中药色谱指纹图谱相似度评价软件2004AB"对其质量进行评价,同时对黄酮碳苷类成分进行主成分分析。结果建立了铁皮石斛叶黄酮碳苷类成分HPLC指纹图谱,标定了9个共有色谱峰,并采用对照品指认了8个色谱峰。3批同属不同种石斛叶与铁皮石斛叶对照图谱明显不同。主成分分析结果表明,黄酮二碳糖苷类成分中芹菜素-6-C-α-L-阿拉伯糖-8-C-β-D-木糖苷对其质量影响最为显著。结论所建立的铁皮石斛叶黄酮碳苷类成分指纹图谱特征性强,方法简便,结合主要成分含量测定可更好地控制其质量,对铁皮石斛叶的鉴定及质量控制具有指导意义和参考价值。
OBJECTIVE To establish the HPLC fingerprints of flavone C-glycosides in Dendrobium officinale leaves and determine the content of apigenin-6-C-α-L-arabinoside-8-C-β-D-xyloside for the quality control METHODS The compounds were separated by using XB C,s (4. 6 mm ×250 mm,5 μm) analytical column. The mobile phase consisted of methanol and water containing 0.4% formic acid. Gradient elution program was used. The detection wavelength was 335 rim. In total 14 batches of Dendrobium officinale leaves and 3 batches of different species from Dendrobium were analyzed. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004AB) and principal component analysis (PCA) were used in data analysis. RESULTS The HPLC fingerprint of flavone C-glycosides of Dendrobium officinale leaves was established. In total 9 peaks were selected as the characteristic common peaks and 8 of them were identified. There were significant differences in the fingerprint chromatograms between Dendrobium officinale leaves and different species from Dendrobium. Principal component analysis showed that apigenin-6-C-α-L-arabinoside-8-C-β- D-xyloside among the flavone di-C-glycosides was the most important component. CONCLUSION The HPLC fingerprint and content of major component can be applied for identification and quality control of Dendrobium officinale leaves.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2012年第11期889-893,共5页
Chinese Pharmaceutical Journal
基金
国家"十二五"科技支撑计划(2011BA104B06)
浙江省大型科学仪器设备协作共用平台科技计划项目(2009F70034)
浙江省高等学校优秀青年教师资助项目[浙教办高科(2010)175号]
浙江省第一批重大科技专项重点项目(2006C13014)
浙江省教育厅科研计划项目(Y200804797)