摘要
目的探讨系统性红斑狼疮(SLE)患者血清基质金属蛋白酶9(MMP-9)及其抑制物(TIMP-1)的表达特点及临床意义。方法用双抗体夹心酶联免疫吸附试验(ELISA)检测64例SLE患者(SLE组)和25名健康人(对照组)血清MMP-9和TIMP-1的水平并进行比较分析。结果 SLE组患者血清MMP-9水平明显低于对照组,TIMP-1明显高于对照组(P<0.01),且MMP-9/TIMP-1低于对照组(P<0.05);SLE患者活动期血清MMP-9、MMP-9/TIMP-1明显低于缓解期(P<0.05),但TIMP-1的水平差异无统计学意义(P>0.05);狼疮肾炎组MMP-9低于非肾炎组(P<0.05),但TIMP-1差异无统计学意义(P>0.05);MMP-9及TIMP-1水平在LN各病理类型患者中的差异无统计学意义(P>0.05);患者的临床表现与MMP-9及TIMP-1水平无明显关系。结论 MMP-9及TIMP-1可能参与SLE的发病,血清MMP-9水平可作为反映SLE活动程度、肾脏损害的指标。
Objective To explore the characteristic of express of serum matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinases-1（TIMP-1）in patients with systemic lupus erythematosus（SLE） and investigate its clinical significance.Methods The levels of serum MMP-9 and TIMP-1 of 64 patients with SLE（SLE group） and 25 healthy（control group） were measured by ELISA.Results The levels of serum MMP-9 of SLE patients were significantly lower than that of the control group,but the TIMP-1 was higher than control group（P0.01）;and the ratio of MMP-9 to TIMP-1 in SLE patients was lower than healthy control group（P0.05）.In active SLE patients,the levels of serum MMP-9 and the ratio of MMP-9 to TIMP-1 were lower than inactive SLE patients;but there was no significant differences of serum TIMP-1 between active SLE patients and inactive SLE patients.The lupus nephritis（LN） patients′serum MMP-9 level was lower than the SLE patients without LN,but there was no significant differences of serum TIMP-1 level between the lupus nephritis patients and the SLE patients without LN（P0.05）.The MMP-9 and TIMP-1 serum levels were no significant differences between different pathological categories.There were no significant relationship between MMP-9,TIMP-1 and clinical manifestations.Conclusion MMP-9 and TIMP-1 may be involved in the pathogenesis of SLE,and the level of serum MMP-9 may be used as an indicator to monitor disease activity,renal damage.
出处
《内科》
2012年第2期101-104,共4页
Internal Medicine
