摘要
分析儿童急性淋巴细胞白血病 (ALL)的免疫表型特点。方法 用一组系列相关单克隆抗体和流式细胞术间接免疫荧光法对 85例儿童ALL进行免疫分型。结果 T系ALL占 2 8.2 % ,B系ALL 6 1.2 % ,T B急性混合细胞白血病 (AMLL) 8.2 % ,其中髓系抗原表达率 2 1.2 % ;各组间L1 、L2 比率、染色体异常及治疗完全缓解 (CR)率均无显著性差异 (P >0 .0 5 ) ;T ALL中CD3平均阳性率较CD2 、CD7低 (P <0 .0 1,P <0 .0 5 ) ,B ALL中CD2 2 阳性率明显低于CD1 0 、CD1 9(P均 <0 .0 0 1)。结论 ALL免疫分型与FAB分型、染色体核型变化无相关性 ;系列特异性单抗CD3、CD2 2 在诊断T ALL、B ALL中起决定作用 ;ALL髓系抗原阳性与治疗CR率无关。
Objective To analyse the immunophenotype of childhood acute lymphoblastic leukemia(ALL). Methods 85 cases of childhood acute lymphoblastic leukemia(ALL) were immunologically classified by a series of lineage-associated monoclonal antibodies and flowcytometric indirect immunofluorescence. Result Percentage of T-ALL was 28.2%, while that of B-ALL and T/B acute mixed-lineage leukemia (AMLL) was 61.2% and 8.2% respectively.The rate of myeloid antigen expression was 21.2% among them. There was no significant difference in rate of L 1、L 2,chromosome abnormality and complete remission (CR) between groups (P>0.05).The positive rate of CD 3 in T-ALL was lower than that of CD 2 and CD 7 (P<0.01,P<0.05);the positive rate of CD 22 in B-ALL was lower than that of CD 10 and CD 19 (P<0.001). Conclusion Immunophenotype was not correlated with FAB subtype or chromosome abnormality in childhood ALL;the lineage-specific monoclonal antibodies CD 3 and CD 22 were important in diagnosing T-ALL and B-ALL.Myeloid antigen expression was not associated with CR rate.
出处
《实用儿科临床杂志》
CAS
CSCD
2000年第2期67-68,共2页
Journal of Applied Clinical Pediatrics
