摘要
目的:建立小鼠组织中KS0604的定量分析方法。方法:采用组织消化结合基于离子交换和反相分配原理的两步固相萃取法,并采用无胶筛分毛细管电泳技术测定小鼠组织中的KS0604。结果:小鼠肾组织中KS0604在2.0～2000μg·g^(-1)范围内呈良好线性,最低定量限LOQ为2.0μg·g^(-1);其他组织中KS0604在1.3～128μg·g^(-1)范围内呈良好线性,最低定量限LOQ为1.3μg·g^(-1);r>0.999。加样回收率试验RSD<10.82%,精密度试验RSD<2.3%,稳定性试验结果表明KS0604在小鼠组织中的稳定性良好。结论:该方法可用于定量检测小鼠组织中的KS0604。
Objective：To establish a method for the quantitative determination of KS0604 in the mouse tissues.Method：A dual solid phase extraction（SPE） pretreatment method coupling with non-gel sieving capillary electrophoresis（NGCE） was established to determine the content of KS0604 in the tissues of mice.Result：The calibration curve of KS0604 was linear within the concentration range of 2.0-2 000μg ？ g^（-1） in kidney and 1.3-128μg ？ g^（-1） in the other mouse tissues with correlation coefficients（r） above 0.999. The LLOQ was 2.0μg ？ g ^（-1） in kidney and 1.3μg ？ g ^（-1） in the other mouse tissues.The RSD of the sample-adding recovery of KS0604 in mouse tissues was less than 10.82%and the precision RSD of the assay was below 2.3%.KS0604 was stable in mouse tissues. Conclusion：The combined method of solid-phase extraction and NGCE can be used in the detection and quantitative determination of KS0604 in mouse tissues.
出处
《中国药师》
CAS
2012年第5期629-632,共4页
China Pharmacist
关键词
非胶筛分毛细管电泳
反义核酸
组织
小鼠
Non-gel sieving capillary electrophoresis
Antisense nucleic acid
Tissue
Mice
