摘要
目的:建立HPLC法测定跌打片中芍药苷含量的方法。方法:色谱柱:Hibar 250-4.6 C_(18)柱(250 mm×4.6 mm,5μm);流动相:乙腈-0.1%磷酸溶液,梯度洗脱;检测波长:230 nm;流速:1.0 ml·min^(-1)。结果:芍药苷0.058 2～0.727 6μg的范围内与峰面积呈良好的线形关系,r=0.999 6(n=6),平均回收率为98.9%,RSD=0.46%(n=9)。结论:所建立的方法简便、可靠,专属性强,重复性好,可作为跌打片的质量控制方法。
Objective：To establish a method for the determination of paeoniflorin in contusion tablets by HPLC.Method：A Hibar 250-4.6 C_（18） column（250 mm×4.6 mm,5μm）was used.The mobile phase was acetonitril-0.1%H_3PO_4 solution with gradient elution. The detection wavelength was at 230 nm and the flow rate was 1.0 ml·min^（-1）.Result：Paeoniflorin showed excellent linear relationship within the range of 0.058 2-0.727 6μg（r =0.999 6,n =6）.The average recovery was 98.9%with RSD of 0.46%（n =9）. Conclusion：The method is simple,reliable,specific and reproducible.It can be used for the quality control of contusion tablets.
出处
《中国药师》
CAS
2012年第5期678-679,共2页
China Pharmacist
