摘要
目的探讨热休克转录因子1（HSFl）蛋白在人肝细胞癌组织中的表达及临床意义，并推测HSF1在肝细胞癌发生、发展中的可能分子机制。方法收集2006年3月至2007年3月第四军医大学西京医院67例行手术切除的肝细胞癌组织，以及21例因肝血管瘤行手术切除的部分正常肝脏组织，采用免疫组织化学法检测肝细胞癌及正常肝脏组织中HSF1蛋白及热休克蛋白70（ttSP70）的表达，分析HSF1蛋白与患者临床病理特征及预后之间的关系，并根据HSF1蛋白与HSP70表达情况的相关性分析推测HSF1在肝细胞癌发生、发展中的可能分子机制。不同组织中HSF1蛋白阳性率的分析以及肝细胞癌中HSF1蛋白阳性表达率与各临床病理资料的关系采用×2检验及Fisher确切概率法，HSF1蛋白与HSP70在肝细胞癌中表达的相关性采用Spearman相关性分析。采用Kaplan—Meier法绘制生存曲线，生存情况比较采用Log—rank检验。结果HSF1蛋白在肝细胞癌组织中的阳性率为69％（46／67），在正常肝脏组织中的阳性率为29％（6／21），两者比较，差异有统计学意义（X。=10．628，P〈0．05）。HSP70在肝细胞癌组织中的阳性率为57％（38／67），在正常肝脏组织中的阳性率为24％（5／21），两者比较，差异有统计学意义（X2=6．929，P〈0．05）。HSFl蛋白与HSP70在肝细胞癌组织中的表达呈正相关（r=0．319，P〈0．05）。HSFl蛋白的高表达与肝细胞癌的包膜完整与否、分化程度以及TNM分期有关（x1=5．935，9．762，5．159，11．267，P〈0．05）；而与患者性别、年龄、HBsAg水平、AFP水平以及是否存在门静脉癌栓无关（x2=0．822，0．172，2．059，P〉0．05）。HSF1蛋白阳性和阴性表达者的生存时间分别为（21．4±1．9）个月和（29．8±2．7）个月，两者生存率比较，差异有统计学意义（X2=4．276，P〈0．05）。结论HSFl与肝细胞癌的发生、发展、侵袭转移以及不良预后相关，其可能通过调控HSP70的表达而发挥作用，在肝细胞癌的诊断和治疗中有良好应用前景。
Objective To investigate the expression and clinical significance of heat shock transcription factor 1 （HSF1） protein in human hepatocellular carcinoma （HCC） tissues, and deduce the probable molecular mechanism of HSF1 in the development and advancement of HCC. Methods Sixty-seven samples of HCC tissue and 21 samples of normal liver tissue were obtained from March 2006 to March 2007 at the Xijing Hospital. The expressions Of HSF1 protein and heat shock protein 70 （HSP70） were detected by using immunohistochemistry. The probable molecular mechanism of HSF1 in the development and advancement of HCC was deduced according to the relationship between the expressions of HSF1 protein and HSP70. Positive rates of HSF1 protein in different tissues and the relationship between HSFI protein expression in the HCC tissues and clinical pathological factors were analyzed by the chi-square test and by calculating Fisher exact probability, respectively. The correlation between the expressions of HSF1 protein and HSP70 in the HCC tissue was analyzed by the Spearman correlation coefficient. The survival curve was drawn by the Kaplan-Meier method, and the survival rate was analyzed by the Log-rank test. Results The positive rates of HSF1 protein expression was 69% （46/67） in the HCC tissue, which was significantly higher than 29% （6/21） in the normal liver tissue （ X2=10. 628, P 〈 0.05 ）. The positive rates of HSP70 expression in the HCC tissue was 57% （38/67）, which was significantly higher than 24% （5/21） in the normal liver tissue （X2 = 6. 929, P 〈 0. 05 ）. The expression of HSF1 protein in the HCC tissue was positively correlated with that of HSPTO （r = 0. 319, P 〈 0. 05 ）. The high expression of HSF1 protein was correlated with the integrity of capsule of HCC, tumor differentiation and TNM stage （X2 = 5. 935,9. 762, 5. 159, 11. 267, P 〈 0.05 ）, while the high expression of HSF1 protein was not correlated with the gender, age, levels of hepatitis B surface antigen and alpha fetoprotein, and portal vein tumor thrombus （X2 = 0. 822, 0. 172, 2. 059, P 〉 0. 05 ）. The survival time was （21.4±1.9）months for patients with positive HSF1 protein expression and （29.8±2.7 ） months for patients with negative HSF1 protein expression. There was a significant difference in the survival time between patients with positive and negative HSF1 protein expression （X2 = 4. 276, P 〈 0.05 ）. Conclusions HSF1 is correlated with the development, advancement, invasion, metastasis and malignant prognosis of HCC. HSF1 takes effects by regulating the expression of HSP70, and it has a good perspective of clinical application for the diagnosis and treatment of HCC.
出处
《中华消化外科杂志》
CAS
CSCD
北大核心
2012年第3期279-283,共5页
Chinese Journal of Digestive Surgery
基金
国家自然科学基金（30971508）
关键词
肝肿瘤
热休克转录因子1
热休克蛋白70
免疫组织化学
Liver neoplasms
Heat shock transcription factor 1
Heat shock protein 70
lmmuno-histochemistry
