摘要
目的建立HPLC法测定益肾养元合剂中补骨脂素和异补骨脂素的含量。方法采用AgilentC18柱(150 mm×4.6 mm,5μm)为色谱柱;流动相为甲醇∶水(35∶65);检测波长为246 nm;流速1.0 ml/min;柱温35℃。结果补骨脂素在40.04～800.80 ng范围内有良好的线性关系(r=0.999 95),平均回收率为98.3%,RSD为2.45%;异补骨脂素在40.88～817.60 ng范围内有良好的线性关系(r=0.999 96),平均回收率为99.1%,RSD为2.16%;结论本法简单、准确可靠,可用于该制剂的质量控制。
Objective To esablish a HPLC method for determining the content of psoralen and isopsoralen in Yishen Yangyuan Heji.Methods The Agilent C18 column（150 mm×4.6 mm,5 μm） was used.The mobile phase was consisted by methanol-water（35∶65）.The detection wavelength was 246 nm.The flow rate was 1.0 ml/min.column at 35 ℃.Results The lingear range of psoralen was 40.04~800.80 ng（r=0.999 95）.The average recovery was 98.3%,RSD was 2.45%.The lingear range of isopsoralen was 40.88~817.60 ng（r=0.999 96）.The average recovery was 99.1%,RSD was 2.16%.Conclusion The method is simple,reliable,accurate and can be applied to the quality control of the preparetion.
出处
《今日药学》
CAS
2012年第4期219-221,共3页
Pharmacy Today
