摘要
乙烯在植物果实成熟过程中起着重要的作用,ACC合酶(1-aminocyclop ropane-1-carboxylic acid synthase,ACS)和ACC氧化酶(1-aminocyclop ropane-1-carboxylic acid oxidase,ACO)是植物乙烯生物合成途径的限速酶。通过DNA序列分析,以不同果实成熟期的6个甜樱桃品种(Prunus avium L.)为材料,检测ACO基因的多态性。获得甜樱桃ACO基因约1 kb,与桃(P.persica)ACO基因(GenBank登录号:AF532976)序列的同源性达96%,其预测的氨基酸序列与桃、梅(P.mume)、美洲李(P.armeniaca)和欧洲李(P.domestica)等ACO的氨基酸序列同源性超过95%。该片段包括4个外显子和4个内含子,内含子符合GT-AT规律。用DNAMAN进行多序列比对分别在内含子2和内含子4内发现2个多态性简单重复序列(AT)n。内含子2有3种片段:即(AT)6、(AT)7和(AT)8;内含子4有2种片段,即(AT)5和(AT)6,组合后共得到4种ACO单倍型。研究在甜樱桃ACO基因座上发现2个SSR标记,为进一步研究ACO基因多态性与果实成熟期相关性奠定基础。
Ethylene plays an important role in ripening of plants. ACC synthease （ 1-aminocyclop ropane- 1-carboxylic acid synthase, ACS） and ACC oxidase （1-aminocyclop ropane-1-carboxylic acid oxidase, ACO） are rate-limiting enzymes in ethylene biosynthesis in plants. DNA sequence analysis was applied to detect the polymorphism of A CO gene in 6 sweet cherry varieties （Prunus avium L.） of different maturity time. The DNA sequences of partial A CO gene about 1 kb were obtained from sweet cherry （P. avium）. The identity of these DNA sequences was 96% with A CO of peach （P. persica）. The identity of predicted amino acid sequences was 95% with the amino acid sequence of ACO in P. persica, Prunus mume, Prunus armeniaca, and Prunus domestica. These fragments contained four extrons and four introns which has GT-AG sequence. Two SSR markers were obtained by nucleotide sequences alignment of A CO genes by DNAMAN and detected in intron 2 and intron 4 separately. Three ACO microsatellite alleles namely （AT）6, （AT）7 and （AT）8 were identified in intron 2. Two ACO microsatellite alleles namely （AT）5 and （AT） 6 were identified in intron 4. Four ACO haplotypes were obtained in sweet cherry. This resuh pro- vided a scientific basis for studying polymorphism of A CO gene and their association with the diversity of maturity time in sweet cherry.
出处
《果树学报》
CAS
CSCD
北大核心
2012年第3期359-365,共7页
Journal of Fruit Science
基金
公益性行业(农业)科研专项经费(200903019)
北京市科技新星计划(2010B025)
北京市农林科学院科技创新能力建设专项(KJCX201102003)
