摘要
僵蚕提取物对棉铃虫有胃毒作用,对饲毒后不同时间棉铃虫3龄幼虫体内碱性磷酸酶的活性进行了研究。结果表明,在3龄棉铃虫人工饲料中添加僵蚕提取物浓度为3mg·g-1时,饲毒第3天试虫的校正死亡率为66.73%;5mg·g-1的剂量导致试虫死亡率可以高达83.33%,表明僵蚕提取物对棉铃虫具有很强的胃毒作用。试虫碱性磷酸酶活性分析表明,1～5mg·g-1浓度处理组的试虫碱性磷酸酶活性与对照组相比均显著下降,并有明显的剂量相关性。推测僵蚕对棉铃虫幼虫的毒杀作用机制之一与其对碱性磷酸酶活性的抑制有关。
This paper reported the study results on feeding toxicity and inhibition effects on alkaline phosphatase（ALP） of Helicoverpa armigera treated with the extracts of Bombyx batryticatus.The corrected mortality of H.armigera treated with the extracts of B.batryticatus at 3 mg·g^-1 concentration in insect diet was 66.7% after feeding on the toxic diet three days,while the corrected mortality was up to 83% to the larvae treated with a concentration of 5 mg·g^-1,indicating that the B.batryticatus extracts was high toxic to the third instar larvae of H.armigera.The enzyme assay showed that the ALP activities decreased significantly while treated with the B.batryticatus extracts concentration ranged from 1 mg·g^-1 to 5 mg·g^-1,and the correlationship was responsible to the dosage of B.batryticatus extracts.Thus,the inhibition on ALP activity is a possible mechanism for the pesticidal activity of B.batryticatus extracts.
出处
《西北林学院学报》
CSCD
北大核心
2012年第3期101-104,共4页
Journal of Northwest Forestry University
基金
公益性行业(农业)科研专项(200903042)
西北农林科技大学基本科研业务费(cx200907,py200903)
关键词
僵蚕
棉铃虫
胃毒作用
碱性磷酸酶
酶活性抑制
Bombyx batryticatus
Helicoverpa armigera
feeding toxicity
alkaline phosphatase
inhibition on enzyme activity
