摘要
目的 :立足于痰浊凝结为癌瘤形成与转移的重要病理因素之一 ,设计观察了消痰散结方 (半夏、胆南星、茯苓、枳实、陈皮、炙甘草等组成 )对裸鼠胃腺癌体内生长的抑制作用及其对胃癌组织中增殖细胞核抗原 (PCNA)表达的影响。方法 :建立裸鼠MKN - 4 5胃腺癌模型 ,采用免疫组化ABC法检测胃癌组织中PCNA的阳性表达率 ,同时观察了不同用药组瘤组织重量和病理学变化。结果 :在裸鼠体内实验观察中 ,消痰散结方组抑瘤率大于 30 % ,其瘤重明显低于空白对照组 (P <0 .0 5) ;与化疗组比较 ,无统计学差异(P >0 .0 5)。中药组裸鼠胃癌组织PCNA表达水平明显低于空白对照组 (P <0 .0 5) ,表明细胞增殖活性降低。结论 :消痰散结方可抑制裸鼠胃癌的生长 。
To observe the effect of Xiaotan Sanjie Fang(XSF)on the growth of gastric adenocarcinoma and the PCNA expression in rats,MKN-45 gastric adenocarcinoma rat model was established.The positive rate of PCNA expression in the cancer tissue was assessed by ABC technique,and pathologic changes of different groups were examined.The results showed that the tumor inhibition rate in XSF group is higher than 30%,and the tumor weight is obviously lower than the control group(P<0.05),but similar to chemotherapy(P>0.05).PCNA expression in XSF group is obviously lower than the control group.It is concluded that XSF can inhibit the growth of gastric cancer and reduce the proliferation and activity of cancer cell.
出处
《广州中医药大学学报》
CAS
2000年第2期152-154,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
关键词
消痰散结方
胃肿瘤
腺癌
中医药疗法
PCNA
XIAOTAN SANJIE FANG/pharmacology
STOMACH NEOPLASMS/TCD therapy
ELIMINAT DAMPNESS RESOLV PHLEGM
@PCNA (original article on page 152)
