摘要
目的筛选对超广谱β-内酰胺酶(ESBLs)的敏感抗生素,为提高ESBLs感染患者临床治愈率提供指导。方法血液感染患者用BacT/Alert全自动血培养仪细菌培养、VitekⅡ细菌鉴定仪进行鉴定,K-B药敏纸片测定法进行药敏试验并筛选出ESBLs细菌160株,肉汤稀释法测定最低抑菌浓度(MIC)。结果 ESBLs菌以大肠埃希菌为主,对亚胺培南、美罗培南、头孢美唑、替加环素、黏菌素和阿米卡星的敏感性分别为100%、100%、94.38%、100%、100%和95.63%,而对头孢他啶和四代头孢菌素的头孢吡肟的敏感率仅达58%,加酶抑制剂的复合抗生素敏感性分别为哌拉西林/三唑巴坦、头孢哌酮/舒巴坦、替卡西林/克拉维酸和氨苄西林/舒巴坦,敏感率分别为82.50%、80.63%、13.13%和3.75%。结论治疗ESBLs感染应以抗生素敏感性试验为指导,提高救治率。
Objective To select the sensitive antibiotics for againsting ESBLs bacterium and to provide guidance for treatment for patients infected ESBLs bacterium. Methods The blood from infective patients was cultured by BacT/Alert automatic blood culture machine and positive bacteria was indentified by VitekII. Furthermore, ESBLs-producing Enterobacteriaceae was selected by Kirby-Bauer and Broth dilution method. Results ESBLs- producing Enterobacteriaceae was distributed in Escherichia coli. The sensitive rate to imipenem/cilastatin, meropenem, cefmetazole, tigecycline, colistin and amikacin of ESBLs-producing Enterobacteriaceae was 100%, 100%, 94.38%, 100%, 100% and 95.63%, respectively. However, the sensitive rate to ceftazidime and cefepime was only 58%. The sensitive rate was different in complex antibiotics which was 82.50%, 80.63%, 13.13% and 3.75% in piperacillin-tazobactam, cefoperazone-sulbactam, ticarcillin/clavulanate and ampicillin/sulbactam. Conclusions The treatment for ESBLs-producing Enterobacteriaceae should select antibiotics according to the result of sensitivity test to improve the survival rate.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2012年第5期388-391,共4页
Chinese Journal of Antibiotics
基金
军队超级细菌紧急课题"多重耐药菌的监测及药物筛选研究"(10YJ003)
关键词
血流感染
超广谱Β-内酰胺酶
药物敏感性
Blood infection
Extended-spectrum β-lactamase
Antibiotics sensitivity
