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比色法测定大蒜多糖提取物中总多糖含量 被引量:12

Content Determination of Total Polysaccharide in the Garlic Polysaccharide by Colorimetry
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摘要 目的:建立大蒜多糖提取物中总多糖的含量测定方法。方法:以葡萄糖为对照品,硫酸-苯酚比色法测定大蒜多糖提取物中总多糖含量。结果:样品经苯酚-硫酸显色后于485 nm处测定吸收度,在此波长处溶液的吸光度与葡萄糖含量呈良好的线性关系;线性范围0.0116~0.0578 g·L-1(r=0.999 5),加样回收率101.4%,RSD 2.1%。结论:该方法简单,准确性、重复性良好,可作为大蒜多糖提取物中总多糖质量的控制方法。 Objective: To develop a method for content determination of total polysaccharide in garlic polysaceharide. Method: Glucose was used as a reference substance, and phenol-sulfuric acid was applied as coloring reagent to determine the content of total polysaccharide. Result: The calibration curve of glucose showed good linearity in the range of 0. 011 6-0. 057 8 g · L-1 (r =0. 999 5). The average recovery rate was 101.4% , and RSD was 2. 1%. Conclusion: The determination method is simple, sensitive and accurate, which can be used for evaluating the quality of total polysaccharide in the garlic polysaccharide.
出处 《中国实验方剂学杂志》 CAS 北大核心 2012年第10期158-161,共4页 Chinese Journal of Experimental Traditional Medical Formulae
基金 "重大新药创制"科技重大专项平台子课题(2009ZX09301-005-11)
关键词 大蒜多糖提取物 总多糖 葡萄糖 比色法 garlic polysaccharide total polysaccharide glucose colorimetry
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