摘要
通过简单厌氧培养方法,共从猪消化道中分离得到10株生长良好的乳酸菌。经菌落形态、细胞形态、生化反应实验、16S rDNA测序,最终确定5株为植物乳杆菌,4株为粪肠球菌,1株为鹑鸡肠球菌。除了粪肠球菌M462和M15对·OH几乎没有清除能力,10株乳酸菌均具有抗氧化活性。多数乳酸菌对·OH、DPPH自由基和O2-·的清除率和细胞浓度呈正相关。在细胞浓度为5×108CFU/mL时,10株乳酸菌对·OH、DPPH自由基和O2-·的最高清除率分别为12.2%、80.6%和79.3%。5株植物乳杆菌对DPPH的清除率都大于60%,好于粪肠球菌;而粪肠球菌对O2-·的清除率好于植物乳杆菌,4株粪肠球菌对O2-·的清除率都大于60%;植物乳杆菌R17、R53和R762对反应体系中的·OH清除率大于5%,其中R17的清除率最高。
Ten strains of lactic acid bacteria（LAB） were isolated from the digestive tract of pigs by anaerobic culture.Based on morphological,physiological,biochemical tests and 16S rDNA sequence analysis,five strains were identified as Lactobacillus plantarum,four strains as Enterococcus faecalis,and stain m661 as Enterococcus gallinarum.All ten strains had strong radical-scavenging capacity although E.faecalis M462 and L.plantarum M15 had almost no scavenging capacity against hydroxyl free radicals.The radical-scavenging capacity of most of the ten strains was positively correlated with bacterial density.At a concentration of 5 × 108 CFU/mL,the highest radical-scavenging rates against hydroxyl,superoxide anion and DPPH free radicals were 12.2%,80.6% and 79.3%,respectively.Five L.plantarum strains revealed a DPPH scavenging rate of higher than 60%,which was significantly higher that that of four Enterococcus faecalis strains.E.faecalis had better scavenging effect against superoxide anion free radicals than other two species and the scavenging rates of four E.faecalis strains against superoxide anion free radicals were all higher than 60%.L.plantarum strains R17,R53 and R762 revealed a scavenging rate of higher than 5% against superoxide anion free radicals,and R17 was the strongest superoxide anion free radical scavenger among them.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第9期221-225,共5页
Food Science
基金
国家自然科学基金项目(31070005)
关键词
猪
乳酸菌
鉴定
抗氧化作用
pig
lactic acid bacteria
identification
antioxidant effect
