摘要
在水相中合成了硫普罗宁(Tiopronin,TP)修饰的CdTe/CdS量子点(TP-CdTe/CdS QDs)。利用紫外-可见吸收光谱、荧光光谱研究了TP-CdTe/CdS QDs与丝裂霉素(mitomycin C,MMC)的相互作用机理。在pH=7.6的tris-HCl缓冲溶液介质中,TP-CdTe/CdS QDs与MMC相互作用,使TP-CdTe/CdS QDs的荧光发生猝灭,并且QDs的荧光强度与MMC的浓度有良好的线性关系(r=0.999 1),线性范围4.7×10-9～1.2×10-8g/mL,检出限(3σ)为1.4×10-8g/mL。此方法快速简便,用于尿样中丝裂霉素的测定,实验结果令人满意。
Tiopronin （TP） capped with CdTe/CdS quantum dots （TP-CdTe/CdS QDs） was synthesized in aqueous solution. The paper utilized ultraviolet-visible （UV-Vis） absorption spectra and fluorescence spectrometry to study the interactions between TP-CdTe/CdS QDs and mitomycin C （MMC）. With the optimum reaction conditions which was found to be at pH = 7.6 with tris-HC1 buffer solution, the quenched fluorescence intensity of TP-CdTe/CdS QDs was linearly proportional with the concentration of MMC, correlation coefficient r =0. 9991, and the detection limits were in the range of 4. 7×10^-9 - 1.2 × 10^-8 g/mL, and the detection limit（30-） for MMC was 1.4 × 10^-8g/mL. The quantitative determination of MMC is quick, simple and convenient. This method has been applied to the determination of MMC in urine samples with satisfactory results.
出处
《应用化学》
CAS
CSCD
北大核心
2012年第6期705-710,共6页
Chinese Journal of Applied Chemistry
基金
西南大学发光与实时分析重庆市重点实验室资助项目(CSTC
2006CA8006)
