摘要
目的 探讨结核分支杆菌重组 380 0 0抗原 (rMT38)诱导豚鼠产生迟发性超敏 (DTH)反应的能力及影响因素。方法 常规皮肤试验法 :H3 7Rv致敏豚鼠 ,皮试时以生理盐水为阴性对照 ,5IUPPD为阳性对照 ,0 1μg、0 2 μg、0 4μgrMT38分别于背部皮内注射 ,注射后 48h测量红肿或硬结反应横、纵直径 (mm)。皮肤试验轮圈法 :5IUPPD及 0 1μg、0 2 μg、0 4μgrMT38于同一只豚鼠皮试 ,每批用 6只已致敏的豚鼠 ,共三批试验。同样 ,5IUPPD及 0 1μg、0 2 μg、0 4μg混合抗原同上皮试。于注射后 2 4h、48h、72h测量红肿或硬结反应横、纵直径 (mm)。结果 常规皮肤试验法 ,0 4μgrMT38诱导DTH反应总平均直径与 0 1μg、0 2 μg相比 ,差异存在极显著性 (P =0 0 0 9、P =0 0 1) ;0 1μg、0 2 μgrMT38诱导豚鼠DTH与PPD相当 ,统计分析这两种剂量差异无显著性。轮圈法 ,对应剂量rMT38与混合抗原诱导豚鼠DTH效果相似。剂量效应 ,对于rMT380 1μg、0 2 μg剂量较合适 ;而混合抗原 ,0 4μg剂量或更高较合适。观察时间 ,rMT38诱发豚鼠DTH 48h测量较好 ,而混合抗原则为 2 4h。 结论rMT38能诱导豚鼠DTH反应 。
Objective To evaluate the ability and influencing factors that recombinant 38 000 antigen induces delayed type hypersensitivity(DTH) reaction in guinea pigs Methods In one skin test, physiological saline as negative control and PPD as positive control, guinea pigs were shaved on the back and given intracutaneous injection with 0 1 μg, 0 2 μg, 0 4 μg of recombinant 38 000 antigen Skin reactions were measured 48 h after antigen injection, each measuring two transverse diameters of the induration In the other skin test, each guinea pig was intracutaneously injected in four sites, respectively with 5 IU PPD and recombinant 38 000 antigen (0 1 μg, 0 2 μg and 0 4 μg) or 5 IU PPD and biantigen(combination 38 000 with 16 000 antigen,0 1 μg,0 2 μg and 0 4 μg) Single antigen and biantigen were tested in three separate experiments Each experiment needs six sensitized guinea pigs Skin reactions were measured 24 h, 48 h and 72 h after antigen injection Results DTH responses induced by 0 4 μg were weaker than 0 1 μg and 0 2 μg in 24 h 0 1 μg and 0 2 μg elicited DTH responses similar in strength to that of 5 IU PPD From 0 1 to 0 4 μg, similar dose of recombinant 38 000 antigen and combination antigen elicited similar DTH responses DTH responses induced by recombinant 38 000 antigen had better be measured at 48 h, however that induced by combination antigen at 24 h Conclusions Recombinant 38 000 antigen can induce DTH responses in guinea pigs Dose of antigen is important factor that affect DTH responses in guinea pigs
出处
《中华结核和呼吸杂志》
CSCD
北大核心
2000年第3期161-163,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
全军"九五"医学科研基金! 98m161
关键词
结核分支杆菌
rMT38
迟发性
超敏反应
Mycobacterium tuberculosis
Recombinant 38 000 antigen
Delayed type hypersensitivity
