摘要
在系统优化了电解质溶液的pH、组成、浓度及仪器条件的基础上,建立了一种测定不同来源血竭中龙血素A和龙血素B的毛细管区带电泳(CZE)方法。采用20 kV的分离电压,25℃的毛细管柱温,211 nm的检测波长以及5 s的压力(3 447 Pa)进样时间,在20 mmo l/L的Na2B4O7缓冲溶液(用NaOH调节pH到9.98,含有10%(v/v,下同)乙腈、5.0%乙二醇和1.0%正丁醇)中,龙血素A和龙血素B在15 min内得到了有效分离与检测。方法的线性范围对于龙血素A和龙血素B分别为1.0～100.0 mg/L和0.5～100.0 mg/L。将该方法用于天然血竭及人工诱导血竭中龙血素A和龙血素B的测定,相对标准偏差在0.6%～3.8%之间,加标回收率在95.1%～105.8%之间。方法具有简单、快速、重现性较好和准确度较高的优点,可以用于血竭样品中龙血素A和龙血素B的测定。
A capillary zone electrophoresis method（CZE） for the simultaneous determination of loureirin A and loureirin B was developed based on the optimized conditions of the pH,composition and concentration of the running buffer solution.Loureirin A and loureirin B were separated and determined effectively within 15 min in a running buffer solution of 20 mmol/L Na2B4O7（pH 9.98 adjusted with NaOH solution） containing 10.0%（v/v） acetonitrile,5.0%（v/v） ethylene glycol and 1.0%（v/v） butanol,with the applied voltage of 20 kV,capillary temperature of 25 ℃,detection wavelength of 211 nm,and injection of 5 s at 3 447 Pa.The linear ranges for the determination of loureirin A and loureirin B were 1.00-100 mg/L and 0.50-100 mg/L,respectively.The determination of loureirin A and loureirin B in dragon’s blood from natural and artificial inoculation was performed by the proposed method.The relative standard deviations for the determination of the two constituents in samples were from 0.6% to 3.8%,and the recoveries ranged between 95.1% and 105.8%.The method is simple,rapid and possesses higher reproducibility and efficiency.It can be used for the determination of loureirin A and loureirin B in dragon’s blood.
出处
《色谱》
CAS
CSCD
北大核心
2012年第5期501-506,共6页
Chinese Journal of Chromatography
基金
云南省自然科学基金项目(2008CD066)
云南省科技厅普洱市党政一把手工程项目(2010AE005)
关键词
毛细管区带电泳
龙血素A
龙血素B
血竭
capillary zone electrophoresis（CZE）； loureirin A； loureirin B； dragon’s blood
