摘要
目的:探讨低强度超声对放疗后犬下颌骨微血管的修复作用,为临床防治放射性骨坏死提供实验理论依据。方法:建立下颌骨放射线损伤的动物模型,免疫荧光观察实验Ⅰ组、实验Ⅱ组与对照组CD34的表达。运用低强度超声对其进行治疗,并通过微血管密度(MVD)检测实验Ⅰ组(超声治疗10d,20d,30d)、实验Ⅱ组(未用低强度超声治疗组)与对照组的微血管密度,所得数据进行统计学分析。结果:成功的建立了放射线损伤的动物模型;免疫荧光染色显示对照组CD34呈强阳性表达,实验Ⅰ组呈阳性表达,实验Ⅱ组呈弱阳性表达;MVD检测结果显示实验Ⅱ组呈弱阳性表达,实验Ⅰ组的微血管密度为:10d组(4.04±0.77),20d组(6.22±0.65),30d组(7.13±0.43);实验Ⅱ组为3.85±0.89;与对照组为7.41±0.45。统计结果显示各组之间的差异具有显著性的意义(P<0.05)。结论:低强度超声对放射线损伤后骨组织内的微血管具有较好的修复作用。
Objective： To investigate the repair effect of low intensity pulsed ultrasound （LIPUS） on micrangium in canines with mandibular osteoradionecrosis, and to provide experimental theories for protecting osteoradionecrosis. Methods： Mandibular osteoradionecrosis animal models were established in this study and were subjected to LIPUS treatment. The expressions of CD34 in group I and group II were tested by immunoflurescence. The microvascular density （MVD） of Group I （treated by ultrasound for 10d, 20d and 30d）, Group II and control group were evaluated for further observations, respectively. The obtained results were statistically analyzed. Results： Mandibular osteoradionecrosis animal models were established successfully. It showed that the expression of CD34 in control group was strongest positive, mild positive in group I , and weak positive in group II; The MVD in Group I were 4.04 ± 0.77 （at 10 days）, 6.22 ± 0.65 （at 20 days）, 7.13 ± 0.43 （at 30 days）; Group II was 3.85 ± 0.89; control group was 7.41± 0.45. There were significant differences among groups （P〈0.05）. Conclusion： LIPUS might accelerate the healing of micrangium in bone tissue with osteoradionecrosis.
出处
《中华老年口腔医学杂志》
2011年第6期321-324,共4页
Chinese Journal of Geriatric Dentistry
基金
博士后基金(20100481522)
山东省优秀中青年科学家奖励基金(2011BSB02001)
济南军区总医院院长基金(2010M08)
关键词
低强度超声
放射性骨坏死
微血管密度
Low intensity pulsed ultrasound
osteoradionecrosis
microvascular density
