摘要
用膜片钳全液泡记录方式,本文研究讨论了不同浓度的NdCl_3加入到萝卜液泡膜的内、外两侧时通道电流的变化。外液中加入2.5mmol/NdCl_3和4mmol/LEGTA溶液时,SV电流明显被抑制;当外液中不含EGTA而同时含有CaCl_2,和不同浓度的NdCl_3时,通道电流均被不同程度的增大,随着钕离子浓度的增大,通道电流逐渐降低;而NdCl_3在膜内与SV通道作用机理的研究表明,钕离子浓度高时对通道电流具有抑制效应,浓度低时则促进通道电流。这种稀土的微量促进效应为进一步研究稀土对植物生理活动的影响以及稀土微肥对农作物的增产效果在通道水平上提供了重要的依据。
In this paper we discussed changes of SV- type channel currents of Radish vacuoles successfully by using the whole- vacuolar patch- clamp recording mode when NdCl3 with different concentrations were added to bath solutions and pipette solutions. SV- type currents would increase and threshold potentials of activation would shift towards more negative values with the increase of concentrations of cytosolic Ca^2＋ . When 2.5mmol/L NdCl3 and 4mmol/L EGTA were added to bath solutions, SV- type currents were suppressed remarkably. When bath solutions contained CaCl2 and NdCl3 with different concentrations free of EGTA, we found that cytosolic Nd^3 ＋ had different promoting effects on SV channels. With the increase of concentrations of cytosolic Nd^3 ＋ , SV- type currents got lower and lower. Finally we also studied the effects of luminal Nd^3 ＋ with different concentrations on the channel currents in detail. We found that NdCl3 with higher concentrations had a strong inhibitory effect on SV currents, while NdCl3 with lower con- centrations promoted channel currents. This promoting effect provides an important basis at channel level for researching further the effects of rare earth on physiological activities of plants and the production- increase effects of rare earth fertilizers on crops.
出处
《吕梁教育学院学报》
2004年第3期17-21,共5页
Journal of Lvliang Education College
基金
国家自然科学基金重大项目资助(批准号:29890280)课题
关键词
膜片钳全液泡记录
SV通道电流
外钙
钕离子
whole- vacuolar patch - clamp recording, SV- type channel current, Cytosolic Ca2 ＋ , Nd3 ＋ .
