摘要
目的研究左金丸醇提物对幽门螺旋杆菌感染人胃癌MKN45细胞生长及凋亡的影响,并探讨其作用机制。方法以不同浓度(7.81、15.63、31.25、62.5、125、250、500μg/mL)的左金丸醇提物分别作用于幽门螺旋杆菌感染的人胃癌MKN45细胞,24、48、72h后用Western blot法检测不同浓度的左金丸醇提物作用于幽门螺旋杆菌感染人胃癌MKN45细胞时凋亡相关蛋白Bax、聚腺苷二磷酸核糖聚合酶(PARP)的表达情况。结果幽门螺旋杆菌感染MKN45细胞组、左金丸醇提物低剂量(40μg/mL)组、左金丸醇提物高剂量(180μ/mL)组凋亡细胞百分比分别为:(0.28±0.105)%、(3.27±0.702)%、(7.7±0.721)%,组间比较差异有统计学意义(P<0.05),凋亡率呈剂量依赖性;Western blot检测结果显示,左金丸醇提物低、高剂量作用幽门螺旋杆菌感染MKN45细胞24h后,PARP蛋白(89×103)、Bax蛋白表达明显增强,凋亡率上升,并呈现剂量依赖性。结论左金丸醇提物能抑制幽门螺旋杆菌感染人胃癌MKN45细胞的增殖并诱导细胞发生凋亡。调控凋亡相关基因Bax、PARP的表达可能是其诱导幽门螺旋杆菌感染的人胃癌MKN45细胞凋亡的分子机制之一。
Objective To study the influence of Zuojinwan alcohol extracts on proliferation and apoptosis of human gastric canc- er cells MKN45 infected by Helicobacter pylori（H, pylori） and to investigate their mechanism. Methods The different concentrations of Zuojinwan alcohol extracts （7.81,15.63,31.25,62.5,125,250,500μg/mL） acted on human gastric cancer cells MKN45 infected by H. pylori respectively. After 24,48,72 h, the cell proliferation activity and apoptosis were detected by MTT and FCM respectively,and the expression of Bax and PARP was detected by Western blot. Results FCM results showed that the percentage of apoptotie cells of MKN45 cells infected by H. pylori,Zuojinwan alcohol extracts low dose （40 μg/mL） and Zuojinwan alcohol extracts high dose（180μg/mL） were （0.28±0. 105）%, （3.27±0. 702）% and （7.7±0. 721）% ,with statistical difference between any two groups（P〈0.05）. The apoptosis rate was dose-dependent. Western blot results showed that after 24 h action of high and low doses of Zuojinwan alcohol extracts on MKN45 cells, the expression of PARP（89 KD） and Bax protein was significantly in- creased in a dose-dependent manner. Conclusion Zuojinwan alcohol extracts can inhibit the proliferation of human gastric cancer cells MKN45 infected by H. pylori and induce apoptosis. Regulation of apoptosis-related genes Bax and PARP expression may be one of the molecular mechanisms for inducing apoptosis of human gastric cancer cell MKN45 infected by H. pylori.
出处
《重庆医学》
CAS
CSCD
北大核心
2012年第15期1462-1464,F0003,共4页
Chongqing medicine
基金
国家自然科学基金资助项目(81072955)
上海市卫生局科研基金资助项目(2010019)
上海市卫生局青年基金资助项目(2011001)
上海市重点学科基金资助项目(S30302)
关键词
左金丸醇提物
幽门螺旋杆菌
胃癌细胞
增殖
凋亡
Zuojinwan alcohol extracts
H. pylor~ human gastric cancer cell
proliferation
apoptosis
