摘要
Bovine serum albumin（BSA） was utilized to assemble with the binary complexes of poly（vinylpyrrolidone）-graft-poly（2- dimethylaminoethyl methacrylate）（PVP-g-PDMAEMA）/DNA formed by layer-by-layer electrostatic interactions to screen the residual surface positive charges of complexes.The coating of BSA was able to decrease the zeta potential of binary complexes nearly to electroneutrality without interfering with DNA condensation ability.The ternary complexes of BSA/PVP-g-PDMAEMA/ DNA demonstrated lower cytotoxicity compared with the binary complexes and also maintained high gene transfection efficiency in HepG2 cells.
Bovine serum albumin（BSA） was utilized to assemble with the binary complexes of poly（vinylpyrrolidone）-graft-poly（2- dimethylaminoethyl methacrylate）（PVP-g-PDMAEMA）/DNA formed by layer-by-layer electrostatic interactions to screen the residual surface positive charges of complexes.The coating of BSA was able to decrease the zeta potential of binary complexes nearly to electroneutrality without interfering with DNA condensation ability.The ternary complexes of BSA/PVP-g-PDMAEMA/ DNA demonstrated lower cytotoxicity compared with the binary complexes and also maintained high gene transfection efficiency in HepG2 cells.
基金
the support of National Natural Science Foundation of China(No31100722)
Specialized Research Fund for the Doctoral Program of Higher Education(No20090032120013)
