摘要
采用五株不同类型的细胞系观察了刀豆蛋白A(Con A)诱导细胞对其增殖的抑制作用。研究发现MLA 144及K562抑制率高可作为检测Con A诱导细胞活性的反应细胞。ConA诱导细胞的抑制作用不受消炎痛影响,但抑制活性可被抗T11单克隆抗体和补体消除,表明ConA诱导细胞来自T11抗原阳性的T细胞群。比较正常人及非何杰金氏淋巴瘤病人ConA诱导细胞的抑制活性。淋巴瘤病人的ConA诱导细胞及其上清液的抑制活性明显低下,加入外源性白细胞介素2可增强正常人和淋巴瘤病人的ConA诱导细胞抑制活性。
Using five different cell lines as reactive cells, the proliferative inhibitory effect of Con A induced suppressive cells (CISC) was observed. The percentage of inhibition of MLA144 was 93.1%, K.562 77.5%, Raji 62.5%, Molt-446.4%, and P388 25.9%. It is possible that K562 and MLA144 can be used as reactive cells instead of the peripheral blood mononuclear cells. Supernatants of CISC were also shown to be inhibitory, although less so than CISC. The inhibitory activity of CISC did not seem to be related to prostaglandin. However, its activity could be diminished by anti-Til monoclonal antibody and complement. These results indicate that CISC is a Til-positive lymphocyte population.The inhibitory activity of CISC from 48 normal donors was determined by this modified method and compared with that of 22 patients with non-Hodgkin's lymphoma. The CISC activity of the patients was significantly lower than that of the normal subjects. Addition of exogeneous IL-2 to the culture enhanced the inhibitory activity in both groups
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1990年第2期131-136,共6页
Acta Academiae Medicinae Sinicae
关键词
ConA诱导细胞
消炎痛
淋巴瘤
Con A induced cells indomethacin lymphoma interleukin 2 anti-Til monoclonal antibody
