摘要
目的研究当归藤中总皂苷的最佳提取工艺。方法采用紫外分光光度法,利用正交实验,考察了溶剂浓度、液料比、提取时间、提取次数对当归藤中总皂苷提取量的影响。结果当归藤中总皂苷的最佳提取工艺为60%乙醇、液料比8∶1、每次2 h、提取4次。结论该工艺简单易行,灵敏度高,是当归藤中提取总皂苷的有效途径。
Objective To optimize the extraction technique of total saponins in Embelia parviflora Wall. Methods Orthogonal test was carried out to investigate the effects of concentration of solvent, the ration of liquid to material, time of extraction, and frequency of extraction on extraction results of total saponins from Embelia parviflora Wall with UV spectrophotometry. Results The optimum extraction technique was extracted Embelia parviflora Wall in 60% ethanol with the ratio of liquid to material at 8 ： 1 for 4 times, with 2 hours for each time. Conclusion The optimized technique is simple and feasible, which can provide reference for further research of Embelia parviflora Wall..
出处
《时珍国医国药》
CAS
CSCD
北大核心
2012年第5期1106-1108,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家"973"计划前期研究专项(No.2007CB516805)
广西壮族自治区科技厅应用基础研究(No.0731050)
药用资源化学与药物分子工程教育部重点实验室主任基金(No.CMEMR2011-19)
广西壮族自治区自然科学基金创新团队项目(No.2010GXNSFF013001)
关键词
当归藤
总皂苷
提取工艺
正交试验
Embelia parviflora Wall.
Total saponins Extraction techniques
Orthogonal test
