摘要
通过福林法对液体碱性蛋白酶的活力进行了测定。研究比较了不同温度及pH下的酶促反应对酶活力测定方法的影响,结果显示:酶活力随反应温度的升高表现为先上升后下降,40～50℃时相对较稳定;pH降低对碱性蛋白酶活力有着负面影响。在确定出较优的适合碱性蛋白酶的活力测定方法后,进一步测试了该方法在液体洗涤剂酶活力测定中的应用,发现在洗涤剂中简单地加入酶制剂后,测定酶活力时会出现较大偏差,酶活力值不稳定。因此,在配制加酶液体洗涤剂时,应该对各种因素,例如温度、pH以及洗涤剂中各种成分与酶的相互影响等进行综合考虑。
Activity of liquid alkaline protease was measured by using Fo!in tnethod. Effects of enzymatic reaction under different temperature and different pH on the enzyme activity assay were investigated and compared. Results showed that as the reaction temperature raises, the enzyme activity increases firstly and then declines;while lowering of the pH of the reaction shows negative impact of the alkaline protease activity. After the optimum conditions for alkaline protease activity assay were identified, the application of the assay method in the determination of enzyme activity of enzymed liquid detergent was further tested. It was discovered that if the enzyme was simply added and mixed with the liquid detergent, the measured enzyme activity of the detergent will appear big deviation and an unstable value. This phenomenon indicated that during the formulation of enzymed liquid detergent, many factors such as the temperature, pH, and the mutual influence of components in the detergent should be comprehensively censidered.
出处
《日用化学工业》
CAS
CSCD
北大核心
2012年第3期192-195,共4页
China Surfactant Detergent & Cosmetics
关键词
蛋白酶
活力分析
福林法
液体洗涤剂
protease
protease activity assay
Folin method
liquid detergent
