摘要
为了给腾冲红花油茶（Camellia reticulataf.simpex Sealy）褐斑病的综合防治提供一定的理论依据,通过实地调查与室内分离、鉴定及接种试验,对腾冲红花油茶褐斑病病原菌及其致病性进行了研究。结果表明,红花油茶褐斑病病原为半知菌亚门腔孢纲黑盘孢目（科）的拟盘多毛孢属茶褐斑拟盘多毛孢Pestalotiopsis guepini。该菌种在人工针刺接种条件下均能在大棚内表现出典型症状。并将发病叶片再分离,得到同样的病原。该菌在PDA培养基上菌丝生长温度为10~30℃,最适25℃;产生分生孢子的温度为20~25℃,最适25℃;分生孢子萌发的温度为15~30℃,最适28℃。该病菌在pH4~8条件下均能生长和产孢,产生分生孢子最适pH5~6,分生孢子萌发和菌丝生长最适pH5。该菌对生长势弱或有伤口的植株有很强的致病性,并为红花油茶褐斑病病原学、发病规律及防治技术的系统研究奠定了基础。
The aim was to provide the base for controlling the brown spot in Camellia reticulata f. simpex Sealy. Through field investigation, laboratory separation, identification and inoculation, the pathogen and susceptibility of brown spot disease of the Camellia reticulata f. Simpex Sealy were studied. The results showed phytopathogen was Pestalotiopsis guepini. The plant could be infected by inoculation with needle sting, and the symptom of infection could be observed in the greenhouse condition. The re-separation and identification from the infected leaf proved that it was the same pathogen as its original one. The temperature range for mycelia growth of Pestalotiopsis guepini was from 10 to 30℃, and the optimum temperature was 25℃. The sporulation temperature range was from 20 to 25℃ , and the optimum was 25℃ . For the conidial germination, the temperature range was from 15 to 30℃ , the optimum being 28℃ , and the range of pH was from 4 to 8, the optimum from 5 to 6. Thus, the Pestalotiopsis guepini had high pathogenic susceptibility to those plants with weak growth potential or wound. The study laid the foundation of etiology, infection cycle of and control system for brown spot disease of Camellia reticulata f. simpex Sealy.
出处
《中国农学通报》
CSCD
2012年第13期81-84,共4页
Chinese Agricultural Science Bulletin
基金
国家科技支撑项目"西南高山区油茶丰产栽培技术集成与示范"(2009BADB1B08+02)
云南省高校森林灾害预警与控制重点实验室开放基金"云南高山区油茶病虫害调查
病原鉴定及综合防治研究"(ZK11A106)
国家林业公益行业研究专项"干热河谷木棉纤维人工林培育关键技术研究"(201104034)
国家林业科技推广项目"干旱条件下木棉产业化栽培技术示范推广"(2011-35)
"云南省重点学科森林保护学"(XKZ200905)
"云南省高校干热河谷植被恢复创新团队"
关键词
滇西红花油茶
褐斑病
病原菌
致病性
生物学特性
Camellia reticulata f. simpex Sealy
brown spot
pathogen
susceptibility
biological characteristics
