摘要
目的:探讨抗核抗体(ANA)检测对自身免疫性疾病诊断的指导意义。方法:回顾性分析2010年1月～2010年12月间在我院就诊的患者血清自身抗体的检测结果,599例患者,其中自身免疫性疾病患者313例,非自身免疫性疾病286例,所有患者血清检测ANA,以Hep-2细胞/肝组织为基质的间接免疫荧光法检测ANA。结果:在自身免疫性疾病中,干燥综合征(SS)、类风湿性关节炎(RA)、混合型结缔组织病(MCTD)和系统性红斑狼疮(SLE)中的阳性率分别为73.43%、53.66%、61.04%和90.63%;在非自身免疫性疾病中的阳性率为21.68%,阳性率明显低于自身免疫性疾病组(P<0.01);在自身免疫性疾病组,荧光核型以颗粒型最多见,占47.22%～63.84%。结论:ANA的检测有助于自身免疫性疾病的诊断和筛查。
Objective To explore the sensitivity and correlation on anti-nuclear antibody in the diagnosis of autoimmune disea- ses. Methods The results of auto- antibody detection in serum were retrospectively analyzed for patients visited from Jan to Dec in 2010, among which 313 cases were AID and 286 cases were patients with non- autoimmune diseases. Serum samples of all patients were analyzed by testing anti-nuclear antibody（ANA） with indirect immunofluorescene（IIF）, based on the cultured human epidermoid car- cinoma cells （Hep-2 cell）/liver. Results Among autoimmune diseases ,the positive rate of SS, RA, MCTD and SLE were 73.43%, 53.66% ,61.04% and 90.63% individually; non- autoimmune diseases were 21.68%, less lower than autoimmune diseases （ P 〈 0.01 ）. The fluorescent karyotype was mainly granular pattern . Conclusion Acting as a screening experimental method is helpful for the diagnosis and screening of autoimmune diseases
出处
《放射免疫学杂志》
CAS
2012年第3期299-301,共3页
Journal of Radioimmanology
关键词
抗核抗体
间接免疫荧光法
滴度
anti-nuclear antibody, indirect immunofluorescene, titer
