摘要
目的探讨黄连根茎提取物药根碱对3T3-L1脂肪细胞葡萄糖摄取、脂肪酸氧化的影响及其可能机制。方法采用不同浓度(265.65、53.75、10.75、2.15、0.45μmol/L)药根碱作用于3T3-L1细胞不同时间(12、24、48、72h),MTT法检测药根碱的细胞毒性。以0.45μmol/L药根碱作用于3T3-L1细胞48h后,用[3H]2-DG标记检测胰岛素介导的葡萄糖摄取情况,14C-palmitic标记检测脂肪酸氧化情况,Real-time PCR检测PPARs基因表达情况,Western blotting检测PPARs蛋白表达情况,以PBS代替药根碱作为对照。结果药根碱最佳作用浓度为0.45μmol/L,最佳作用时间为48h。与PBS对照组相比,药根碱具有促进3T3-L1细胞脂肪酸氧化、PPARα、PPARβ基因和蛋白表达的作用(P<0.01或P<0.05),而对PPARγ基因和蛋白表达无明显影响。结论药根碱可显著促进3T3-L1细胞的脂肪酸氧化,其机制可能与上调PPARα、PPARβ基因及蛋白表达有关。
Objective To investigate the effect of jatrorrhizine,extracted from the rhizome of Coptis chinensis,on the glucose up-take and fatty acid oxidation in the 3T3-L1 adipocyte.Methods Jatrorrhizine in different concentrations（265.65,53.75,10.75,2.15 and 0.45μmol/L） was used in culture of the 3T3-L1 adipocytes for different periods（12,24,48 and 72 hours）,and the cytotoxicity of jatrorrhizine was then determined by MTT assay.The uptake of glucose mediated by insulin was assessed by 3H-labeled deoxyglucose,and fatty acid oxidation in co-cultured 3T3-L1 adipocyte was measured using 14C-labeled palmitic acid.The expression of PPARs gene was detected by real-time PCR,and the expression of PPARs protein was detected by Western blotting.Jatrorrhizine was replaced with PBS solution for all the control experiments.Results The optimal active concentration of jatrorrhizine was 0.45μmol/L,and the preferable reaction time was 48 hours.Compared to PBS solution,the in vitro fatty acid oxidation in 3T3-L1 adipocyte was promoted by jatrorrhizine,so were the genic and protein expressions of PPARα and PPARβ（P0.01 or P0.05）.The impact on the PPARγ,however,was negative.Conclusion Jatrorrhizine can promote the fatty acid oxidation in 3T3-L1 adipocyte,which may be attributable to the up-regulation of PPARα and PPARβ levels in 3T3-L1 adipocyte.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2012年第6期585-588,共4页
Medical Journal of Chinese People's Liberation Army
基金
国家科技支撑计划地方专项(2007BA14B03)~~
