摘要
采用SDS/氯仿/苯酚法和oligo(dT)纤维素亲和层析从人胎肝提取poly(A)^+mRNA,注入非洲爪蟾卵母细胞,翻译出肝细胞生长因子(HGF),产物能从卵母细胞中分泌。在9种细胞(包括人与小鼠的4种不同组织和5种细胞系)检测系统中,证明翻译的HGF与直接提取的HGF活性一致,应用滤膜超滤法估计分子量均在10～30kDa,可以初步认为两者是同一物质。从而,支持了人胎肝HGF是胎儿肝脏细胞基因表达的产物。
Poly(A)+ mRNA extracted from human fetal liver by SDS/chloroform/phenol method and oligo(dT) cellulose affinity chromatography was microinjected into Xenopus laevis oo-cytes where it conducted the synthesis of hepatic growth factor(HGF). The translated HGF could be secreted out of the oocytes. The translated HGF by oocytes possessed the essential peculiarities as those of the biochemically purified HGF of the same source, showing similar hepatic stimulating activity and molecular size in the range of 10-30kDa as determined by membrane ultrafiltration technique. It is, therefore, reasonable to conclude that translated HGF is the same substance as biochemically purified one and that HGF of human fetal liver origin is the gene expression product of fetal liver cells.
出处
《中国应用生理学杂志》
CAS
CSCD
1990年第4期298-302,共5页
Chinese Journal of Applied Physiology
基金
国家青年自然科学基金
关键词
胎肝
HGF
爪蟾卵母细胞
体外翻译
human fetal liver
hepatic growth factor
poly(A)+ mRNA
Xenopus laevis oo-cytes
in vitro translation
