摘要
目的:探讨矢车菊素-3-葡萄糖苷对体内外卵巢癌生长的影响及其机制。方法:矢车菊素-3-葡萄糖苷作用人卵巢癌细胞株HO-8910PM后,CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡;Western blotting检测卵巢癌细胞中Mucin-4蛋白表达;建立起裸鼠卵巢癌皮下移植瘤模型,观察矢车菊素-3-葡萄糖苷对裸鼠卵巢癌皮下移植瘤生长的影响;免疫组织化学法检测肿瘤组织中Ki-67和Mucin-4的阳性表达。结果:与对照组相比较,矢车菊素-3-葡萄糖苷可显著抑制体外卵巢癌HO-8910PM细胞生长,并明显诱导细胞凋亡;可显著下调卵巢癌细胞中Mucin-4的表达。矢车菊素-3-葡萄糖苷可显著抑制裸鼠卵巢癌皮下移植瘤生长;可明显降低卵巢癌肿瘤组织中Ki-67和Mucin-4的阳性表达。结论:矢车菊素-3-葡萄糖苷可显著抑制体内外卵巢癌生长,该作用可能通过抑制Mucin-4蛋白表达而实现。
Objective： To investigate the effect and the mechanism of cyanidin-3-glucoside （C3G） in the growth inhibition of ovarian cancer in vitro and in vivo. Method： After human ovarian cancer cell line HO-8910PM was treated with C3G, cell growth was determined by the Cell Counting Kit-8 （CCK-8） assay and apoptosis was evaluated by flow cytometry analysis stained with Annexin V-FITC/PI. The protein expression in HO-8910PM cells was analyzed by Western blot assay. HO-8910PM cells were injected subcuta- neously into nude mice to establish xenograft model. After 3 weeks of implantation, mice were randomized into 2 groups （ n = 8 ） ： con- trol group, feed with 0. 2 mL double distilled water; C3G group, feed with C3G at a dose of 5 mg·kg^-1. All treatment lasted for two weeks, thrice per week. Eight weeks after implantation, tumor weight and inhibition rate were evaluated respectively after the mice were sacrificed. Immunohistoehemistry was used to detect the positive expression of Ki-67 and Mucin-4 in the tumors. Result： The proliferation of ovarian cancer cells was inhibited significantly by C3G with IC50 being 13. 82 mg·L^-1. Apoptosis rate induced by C3G was markedly highter than that of control. The expression of Mucin-4 was down-regulated in HO-8910PM ceils after treatment of C3G. C3G inhibited the growth of ovarian xenograft tumors in nude mice. Furthermore, the positive expression of Ki-67 and Muein-4 were both decreased in tumors after administration of C3G. Conclusion： C3G exerts anti-tumor activity in ovarian cancer both in vitro and in vivo, which may be related to down-regulation of Mucin-4 protein.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第11期1651-1654,共4页
China Journal of Chinese Materia Medica
基金
浙江省中医药管理局重点项目(2008ZA015)
