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人脐带间质干细胞的CM-Dil标记及其移植在急性肾损伤大鼠体内的示踪研究 被引量：1

Human umbilical cord mesenchymal stem cells labeled with CM-Dil and transplanted in acute kidney injury rats for tracer study
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摘要 目的:探讨人脐带间质干细胞(human umbilical cord mesenchymal stem cells,hucMSCs)用CM-Dil染料标记之后生物学特性及体内定位示踪情况。方法:采用脐带组织块贴壁培养法分离人脐带间质干细胞,用CM-Dil染料标记第3代hucMSCs;采用细胞计数法分析CM-Dil标记后hucMSCs的生长变化情况;通过活体成像仪观察CM-Dil染料标记的hucMSCs移植于急性肾损伤大鼠后的体内定位。结果:CM-Dil染料标记hucMSCs的效率很高;标记后的hucMSCs生长增殖能力没有发生明显变化;体内示踪可以观察到标记的hucMSCs。结论:CM-Dil标记不影响hucM-SCs的生长增殖特征,并且能够在体内示踪,为后续研究提供了实验基础。 Objective： To investigate whether the characteristics of human umbilical cord mesenchymal stem cells（hucMSCs） labeled with CM-Dil will be changed;and if CM-Dil labled hucMSCs can be traced in vivo.Methods： HucMSCs were isolated from human umbilical cord.The hucMSCs of passage 3 were selected for labeling in vitro.Using cell counting method to analyse growth curves of CM-Dil labeled hucMSCs;observed CM-Dil labeled hucMSCs in vivo with imaging instrument.Results： The efficiency of CM-Dil dye-labeled hucMSCs was high.The proliferation of labeled hucMSCs had no obvious change.CM-Dil dye-labeled hucMSCs can be observed in vivo.Conclusion： CM-Dil labeled hucMSCs can be traced in vivo.
作者 徐会涛 周颖 王冰莹 毛飞 徐会娟 许文荣 朱伟 钱晖
机构地区 江苏大学基础医学与医学技术学院
出处 《江苏大学学报（医学版）》 CAS 2012年第3期209-212,共4页 Journal of Jiangsu University：Medicine Edition
基金 国家自然科学基金资助项目(31140063) 江苏省博士研究生创新基金资助项目(CX10B_281Z) 江苏大学高级人才启动基金资助项目(07JDG056) 江苏大学科技创新团队和拔尖人才项目(2008-018-02)
关键词 人脐带间质干细胞 CM-Dil染料 体内示踪 急性肾损伤 hucMSCs CM-Dil trace in vivo acute kidney injury
分类号 R33 [医药卫生—人体生理学]
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参考文献8

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同被引文献17

  • 1许剑,徐格林,刘新峰.骨髓基质干细胞的标记示踪技术及其应用[J].中国临床神经科学,2006,14(2):197-201. 被引量：9
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  • 4Yu Y,Cho H I,Wang D, et al. Adoptive transfer of Tc1 or Tc17 cells elicits antitumor immunity against established melanoma through distinct mechanisms[J].J Immunol,2013,190(4):1873-1881.
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江苏大学学报（医学版）
2012年 第3期

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