摘要
首次采用白羊草拔节期茎段为外植体,通过对其组织培养中愈伤组织诱导和植株再生情况进行研究,从而筛选出其在组织培养过程中较合适的激素组合。结果表明,茎段作为外植体时,愈伤诱导在仅附加2,4-D 2.0 mg/L诱导培养基中的诱导率高于2,4-D和6-BA的激素组合;诱导出的愈伤组织转入MSB+6-BA 0.07 mg/L+NAA 0.05 mg/L分化培养基中,分化率显著高于其他激素组合。
Stem segment ofBothriochloa ischaemum as explant, callus induction and planets regeneration was conducted t~ screen a suitable hormone combination. Results showed that the inductivity of Bothriochloa ischaemum stem segment was higher in MSB medium added only 2.0 mg/L 2,4-D than in hormone combination of 2,4-D and 6-BA. The embryo calluses obtained from induction medium could be succeeded on medium MSB ＋ 6-BA 0.07 mg/L ＋ NAA 0.05 mg/L, they had remarkable high differentiation frequency than in other hormone combinations.
出处
《山西农业科学》
2012年第6期575-578,共4页
Journal of Shanxi Agricultural Sciences
基金
国家科技支撑计划项目(2007BAD56B01)
关键词
白羊草
茎段
愈伤诱导
植株再生
Bothriochloa ischaemum
stem segment
embryogenic callus
plant regeneration
